FIGURE

Fig. S2

ID

ZDB-FIG-091106-5

Publication

Zeng et al., 2009 - miR-145 directs intestinal maturation in zebrafish

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Fig. S2

Loss of miR-145 leads to smooth muscle marker expression changes. (A) RT-PCR shows increased expression of nm-mhc-b and smoothelin in miR-145 morphants at 54, 72, and 96 hpf in comparison with the housekeeping gene EF1α. (B) qPCR indicates significantly increased expression of gata6 and sm22α-b but similar level of αsma in 48 hpf embryos treated with pre-miR-145 morpholino. (C) qPCR shows the increased expression of nm-mhc-b in miR-145 MO or pre-miR-145 morpholino treated embryos at 48 hpf. Bars, mean ± SEM. *, P < 0.05.

Expression Data

Genes:	acta2 gata6 myh10 smtnb tagln
Fish:	WT WT + MO1-mir145 WT + MO2-mir145
Knockdown Reagents:	MO1-mir145 MO2-mir145
Anatomical Term:	whole organism
Stage Range:	Prim-5 to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA