FIGURE

Fig. 5

ID

ZDB-FIG-091016-30

Publication

Anderson et al., 2009 - Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration

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Fig. 5

Lack of Dnmt1 activity does not arrest the cell cycle. (A, B) Confocal planes showing EdU incorporation in 84 hpf WT (A) and dnmt1 mutant (B) Tg(ptf1a:EGFP)^jh1; Tg(ins:dsRed)^m1081 larvae. (C, D) Phospho-histone 3 (late G2 interphase through anaphase marker) and Dnmt1 staining of 84 hpf WT and dnmt1^s904 mutant Tg(fabp10:DsRed; ela:GFP)^gz12 larvae. (C′, D′) Dnmt1 channel only. (E) EdU incorporation rate in pancreatic acinar cells, hepatocytes, and intestine of dnmt1 mutants appears indistinguishable from WT (p = 0.87, p = 0.52, p = 0.91, respectively). Error bars = SEM. Significance assessed by Student′s t-test. (F, G) H2BRFP label retention analysis of endodermal organ forming region in WT Tg(gut:GFP)^s854 animals at 52 (F) and 84 (G) hpf. The ventral pancreatic bud (vpb) is demarcated by a dashed line. H2BRFP is diminished in the highly proliferative vpb relative to the dorsal pancreatic bud during outgrowth (F) and pancreas tail formation (G). (F′, G′) H2BRFP (red) and Ins (blue) channels only. Primary islet (pi).

Expression Data

Genes:	dnmt1 DsRed EGFP GFP
Antibodies:	Ab1-dnmt1 Ab7-h3
Fish:	gz15Tg s854Tg jh1Tg; m1018Tg dnmt1^s904/s904; gz15Tg dnmt1^s904/s904; jh1Tg; m1018Tg
Anatomical Terms:	anterior pancreatic bud endocrine pancreas exocrine pancreas intestine pancreas pancreatic duct
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dnmt1^s904/s904; gz15Tg dnmt1^s904/s904; jh1Tg; m1018Tg
Observed In:	cell cycle process exocrine pancreas
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 334(1), Anderson, R.M., Bosch, J.A., Goll, M.G., Hesselson, D., Dong, P.D., Shin, D., Chi, N.C., Shin, C.H., Schlegel, A., Halpern, M., and Stainier, D.Y., Loss of Dnmt1 catalytic activity reveals multiple roles for DNA methylation during pancreas development and regeneration, 213-223, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.