FIGURE

Fig. S4

ID

ZDB-FIG-090710-74

Publication

Aanstad et al., 2009 - The Extracellular Domain of Smoothened Regulates Ciliary Localization and Is Required for High-Level Hh Signaling

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Fig. S4

CLDSmo and CLDSmoΔCRD do not localize to cilia in zebrafish embryos.
Expression of the ciliary marker acetylated Tubulin (green) and Myc-tagged (red) CLDSmo (A) or CLDSmoΔCRD (B) in the dorsal midline in cross-sections of 10 hpf zebrafish embryos. Nuclei were visualized with Hoechst (blue). Like in NIH-3T3 cells, CLDSmo was never detected on the cilium in zebrafish embryos (A). Similarly, the CLD mutations abolished the ciliary localization of SmoΔCRD in zebrafish embryos (B).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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