FIGURE

Fig. 2

ID

ZDB-FIG-090223-3

Publication

Vasilyev et al., 2009 - Collective Cell Migration Drives Morphogenesis of the Kidney Nephron

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Fig. 2

Pronephric Epithelial Cells Migrate toward the Glomerulus

Individual frames of confocal fluorescent time-lapse videos at 2.5-h intervals are presented for five different kidney GFP transgenics. White arrowheads in (A–F) mark individual cells at different time points in migration.

(A) ET33-D10 GFP transgenic (proximal segment).(B) ET11–9 GFP transgenic (mid segment).

(C) CD41:GFP transgenic, multiciliated cells, mid kidney. (D) NaK ATPase:GFP transgenic, all transporting epithelia. (E) ret1:GFP transgenic, distal collecting segment. (F) NaK ATPase:GFP transgenic. Small arrows mark the convolutions forming in the proximal tubules. (G) The rate of migration in (F) is plotted as a function of the distance from proximal-most kidney. (H) Rates of migration determined for various segments of the kidney.

The upper panels in (A), (B), (D), and (E) show live GFP transgenics. The upper panel in (C) shows immunofluorescent image using anti-GFP tagged antibody. The scale bar lengths are as follows: (A), (B), (D), (E): 80 μm, (C): 60 μm, (F): 200 μm. Inter-frame time interval is 2.5 h in (A–E) and 10 h in (F). The rate of migration is measured between 2 and 2.5 dpf in (G) and between 2.5 and 3 dpf in (H).

Expression Data

Genes:	EGFP GFP
Fish:	sqet11Et sqet33D10Et (AB) zf34Tg zf530Tg
Anatomical Term:	pronephros
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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