FIGURE

Fig. 6

ID
ZDB-FIG-090217-2
Publication
Leung et al., 2008 - The orphan G protein-coupled receptor 161 is required for left-right patterning
Other Figures
All Figure Page
Back to All Figure Page
Fig. 6

gpr161 knockdown disrupts Ca2+ handling which is essential for establishment of L–R asymmetry. (A) Defective Ca2+ handling resulting in elevated intracellular Ca2+ in gpr161 knockdown embryos surrounding the Kupffer′s vesicle. Pseudocolour scale for the intracellular Ca2+ signal as normal, intermediate and high intensity. The percentage of embryos exhibited such level of Ca2+ signal was listed in control, gpr161 knockdown, ncx1- and pmca-RNA rescue of the gpr161 knockdown embryos. (B) Removal of excessive Ca2+ by over-expression of 1 pg of Ca2+ pump pmca or Na+/Ca2+ exchanger ncx1 can rescue gpr161 knockdown phenotype. Cardiac looping defect in gpr161-MO (MO#24, 54.1 ± 4.0%; n = 368), rescued by pmca-RNA (32.0 ± 6.0%; n = 238), rescued by ncx1-RNA (34.0 ± 4.8%; n = 144) and results were from 4 injection experiments. n was number of total embryos. Error bars were ± SEM. t-test * and # indicated statistical significance, p < 0.01. All scale bars were 100 μm. (C) A model of gpr161 involved in Ca2+ handling and essential for L–R asymmetry for cardiac morphogenesis and visceral asymmetry in developing zebrafish embryos. Blocking gpr161 function led to defect in Ca2+ handling and resulted in elevated free cytosolic Ca2+ and loss of cardiac looping and abnormal chamber morphogenesis. The gpr161 knockdown can be rescued by removing excessive cytosolic free Ca2+ using over-expression of Ca2+ pump pmca and Na+/Ca2+ exchanger ncx1.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: 10-13 somites

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 323(1), Leung, T., Humbert, J.E., Stauffer, A.M., Giger, K.E., Chen, H., Tsai, H.J., Wang, C., Mirshahi, T., and Robishaw, J.D., The orphan G protein-coupled receptor 161 is required for left-right patterning, 31-40, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.