ZFIN ID: ZDB-FIG-081222-6
Osborne et al., 2008 - The spinster homolog, two of hearts, is required for sphingosine 1-phosphate signaling in zebrafish. Current biology : CB   18(23):1882-1888 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 14-19 somites to 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 14-19 somites to Prim-25

Fig. 1  two of hearts and miles apart Mutant Phenotypes

Comparison of WT (A–C), toh mutant (D–F), and mil/s1p2 mutant (G–I) embryos.

(A, D, and G) Lateral brightfield images, anterior to the left, at 36 hpf show pericardial edema (arrow) and epidermal blisters (insets, arrowheads) in the tails of tohsk12 (D) and mil/s1p2m93 (G) mutants.

(B, E and H) Examination of cmlc2 expression at 19 hpf shows heart-ring formation in WT embryos (B) and a failure in precardiac-mesoderm migration in tohs420 (E) and mil/s1p2m93 (H) mutant embryos. Dorsal views with anterior upwards.

(C, F and I) Visualization of the anterior endoderm by Tg(-0.7her5:EGFP)ne2067 expression at 18 hpf. In embryos injected with toh (F) and mil/s1p2 (I) MOs, numerous gaps (arrowheads) appear in the endodermal sheet, which is also irregularly shaped. The most anterior region of mil/s1p2 morphants lacks GFP+ endodermal cells at the midline (asterisk). Dorsal views with anterior upwards.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ne2067Tg standard conditions 14-19 somites endoderm IFL
ne2067Tg + MO1-spns2 standard conditions 14-19 somites endoderm IFL
myl7 WT standard conditions 20-25 somites heart rudiment ISH
s1pr2m93/m93 standard conditions 20-25 somites heart rudiment ISH
spns2sk12/sk12 standard conditions 20-25 somites heart rudiment ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ne2067Tg + MO1-spns2 standard conditions 14-19 somites endoderm shape, abnormal
14-19 somites endoderm structure, abnormal
s1pr2m93/m93 standard conditions 20-25 somites heart rudiment bifurcated, abnormal
Prim-25 blood circulation disrupted, abnormal
Prim-25 caudal fin endoderm blistered, abnormal
Prim-25 heart edematous, abnormal
spns2sk12/sk12 standard conditions 20-25 somites heart rudiment bifurcated, abnormal
Prim-25 blood circulation disrupted, abnormal
Prim-25 caudal fin endoderm blistered, abnormal
Prim-25 heart edematous, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.