FIGURE

Fig. 6

ID
ZDB-FIG-081219-34
Publication
Gansner et al., 2008 - Essential role for the alpha 1 chain of type VIII collagen in Zebrafish notochord formation
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Fig. 6

Morpholino knockdown of col8a1 recapitulates the gulm208 phenotype. A: Wild-type embryos injected with 12 ng of control morpholino exhibit normal morphology. B: Embryos injected with 2.4 ng of a splice morpholino targeting col8a1 develop notochord distortion (arrowheads) characteristic of gulm208 mutants. C: Reverse transcriptase-polymerase chain reaction (RT-PCR) at 24 hours postfertilization (hpf) demonstrating a dose-dependent reduction in col8a1 transcript after morpholino injection. D: Embryos injected with 12 ng of a splice morpholino targeting col8a1 develop notochord distortion (arrowheads) that is worse than in gulm208 mutants. Insets show notochord at higher magnification. All embryos were treated with PTU to inhibit melanin pigmentation and photographed at 30 hpf.

Expression Data
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Phenotype Detail
Acknowledgments
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