ZFIN ID: ZDB-FIG-081219-34
Gansner et al., 2008 - Essential role for the alpha 1 chain of type VIII collagen in Zebrafish notochord formation. Developmental dynamics : an official publication of the American Association of Anatomists   237(12):3715-3726 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 6 Morpholino knockdown of col8a1 recapitulates the gulm208 phenotype. A: Wild-type embryos injected with 12 ng of control morpholino exhibit normal morphology. B: Embryos injected with 2.4 ng of a splice morpholino targeting col8a1 develop notochord distortion (arrowheads) characteristic of gulm208 mutants. C: Reverse transcriptase-polymerase chain reaction (RT-PCR) at 24 hours postfertilization (hpf) demonstrating a dose-dependent reduction in col8a1 transcript after morpholino injection. D: Embryos injected with 12 ng of a splice morpholino targeting col8a1 develop notochord distortion (arrowheads) that is worse than in gulm208 mutants. Insets show notochord at higher magnification. All embryos were treated with PTU to inhibit melanin pigmentation and photographed at 30 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col8a1a WT control Prim-5 whole organism RTPCR
WT + MO2-col8a1a standard conditions Prim-5 whole organism RTPCR
tbx16 WT control Prim-5 whole organism RTPCR
WT + MO2-col8a1a standard conditions Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-col8a1a standard conditions Prim-15 notochord deformed, abnormal
Prim-15 notochord formation disrupted, abnormal
Acknowledgments:
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