FIGURE

Fig. 7

ID

ZDB-FIG-081202-14

Publication

Munson et al., 2008 - Regulation of neurocoel morphogenesis by Pard6gammab

Other Figures

(all 12)

All Figure Page

Back to All Figure Page

Fig. 7

Role of the Par3/Par6/aPKC complex in apical membrane formation in the neural tube. All images represent transverse sections between the first and sixth somite stained for ZO-1 (blue), aPKC (red), and filamentous actin (green) at 24 hpf. (A) pard6γb^s441 mutant neural tubes show a reduced amount of apical membranes. (B) pard6γb^s441/prkci double mutants show a more severe reduction in apical membrane formation than pard6γb^s441 mutants. (C) pard6γb^s441 mutants injected with a pard3 morpholino show a more severe reduction in apical membrane formation than pard6γb^s441 mutants. (D) mpp5 mutant neural tubes do not show an obvious severe reduction in apical membrane formation. (E) pard6γb^s441/mpp5 double mutants show a more severe reduction in apical membrane formation than pard6γb^s441 or mpp5 single mutants. In addition, pard6γb^s441/prkci and pard6γb^s441/mpp5 double mutants as well as pard6γb^s441 mutants injected with a pard3 morpholino exhibit a severe epithelial disorganization in the neural tube. Scale bars represent 50 μm.

Expression Data

Antibodies:	Ab1-prkca Ab1-tjp1
Fish:	pals1a^m520/m520 pard6gb^s441/s441 pals1a^m520/m520; pard6gb^s441/s441 pard6gb^s441/s441; prkci^m567/m567
Anatomical Term:	neural tube epithelial cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pals1a^m520/m520 pard6gb^s441/s441 pals1a^m520/m520; pard6gb^s441/s441 pard6gb^s441/s441; prkci^m567/m567
Observed In:	central canal neural tube neural tube formation
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 324(1), Munson, C., Huisken, J., Bit-Avragim, N., Kuo, T., Dong, P.D., Ober, E.A., Verkade, H., Abdelilah-Seyfried, S., and Stainier, D.Y., Regulation of neurocoel morphogenesis by Pard6gammab, 41-54, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.