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Fig. 4

ID

ZDB-FIG-081111-26

Publication

Martin et al., 1999 - A role for DNA methylation in gastrulation and somite patterning

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Fig. 4

Global changes in DNA methylation during early zebrafish development. DNA collected from sperm, blastula stage (4 h), gastrula-stage embryos (6 h), somite-stage embryos (16 h), and 24-h embryos were digested with HpaII or MspI, and hybridized to the zebrafish interspersed repetitive sequence DANA/mermaid. Densitometric scans were performed in regions of the gel corresponding to low- and mid-molecular-weight DNA restriction fragments (region between left arrows). Mean density values (indicated here for this particular gel) were used to produce a comparative density ratio between the corresponding HpaII and MspI lanes. A high ratio indicates low levels of DNA methylation (i.e., high similarity between the densities of HpaII and MspI lanes) and low ratio indicates higher levels of DNA methylation. Ratios are indicated as the average of three independent gels ± SD.

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Acknowledgments

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Reprinted from Developmental Biology, 206, Martin, C.C., Laforest, L., Akimenko, M.-A., and Ekker, M., A role for DNA methylation in gastrulation and somite patterning, 189-205, Copyright (1999) with permission from Elsevier. Full text @ Dev. Biol.