Fig. 5

Effect of Il1rapl1b and Il1rapl1b-P455H expression on the axon terminal morphology of olfactory sensory neurons. (A) Omp promoter-driven expression vectors for GAP43-EGFP (Pomp-GG, top), for Il1rapl1b and GAP43-EGFP (Pomp-GG-Il1rapl1b, middle) and for Il1rapl1b-P455H and GAP43-EGFP (Pomp-GG-P455H, bottom) in olfactory sensory neurons. Black boxes, the omp promoter; crosshatched boxes, the 3′ downstream sequence of the omp gene; hatched boxes, SV40 polyadenylation signal sequence; lines, pBluescript II SK+. (B–D) Representative GAP43-EGFP signals in axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-GG (B), Pomp-GG-Il1rapl1b (C) or Pomp-GG-P455H (D) at 36 hpf (left), 60 hpf (middle) and 84 hpf (right). Bar = 5 μm. (E) The area (top), perimeter (middle) and complexity (bottom) values of axon terminals of olfactory sensory neurons in zebrafish embryos injected with Pomp-GG (open bars), Pomp-GG-Il1rapl1b (filled bars) or Pomp-GG-P455H (stippled bars) expression vectors at 36 hpf, 60 hpf and 84 hpf. Pomp-GG, n = 39, 52 and 57 for 31, 38 and 37 embryos at 36 hpf, 60 hpf and 84 hpf, respectively; Pomp-GG-Il1rapl1b, n = 62, 50 and 53 for 35, 36 and 38 embryos at 36 hpf, 60 hpf and 84 hpf, respectively; Pomp-GG-P455H, n = 44, 56 and 45 for 26, 35 and 35 embryos at 36 hpf, 60 hpf and 84 hpf, respectively. Two-way ANOVA: expression vector effect, F = 5.0 and p = 0.0071, F = 9.7 and p < 0.001, and F = 11.2 and p < 0.001 for axon terminal area, perimeter and complexity, respectively. All values represent mean ± SEM. ***, p < 0.001.