FIGURE

Fig. 4

ID

ZDB-FIG-080710-15

Publication

Culp et al., 1991 - High-frequency germ-line transmission of plasmid DNA sequences injected into fertilized zebrafish eggs

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Fig. 4

Identification of a transgenic fish carrying multiple insertions and Mendelian inheritance in the F₂ generation. Southern analysis was performed as in Fig. 3. (A) Plasmid sequences transmitted by founder fish M9. (B) Sequences inherited by individual F₁ fish derived from M9. (C) Sequences present in 18 randomly selected F₂ fish derived from an F₁ fish of the type shown in lanes 5, 6, and 10 of B. For A and B, DNA was derived from offspring pools ofM9 or individual PCR-positive F₁ fish, respectively; while in C, DNA was derived from individual 7-week-old fish. Lanes in B: 1, negative sibling of F1 transgenics; 2-10, F₁ transgenic fish that inherit band 1, band 3, bands 1 and 3, or bands 2 and 4.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA