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ZDB-FIG-080620-34

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Oates et al., 2000 - Too much interference: injection of double-stranded RNA has nonspecific effects in the zebrafish embryo

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Fig. 2

The effects of tbx16/spadetail dsRNA injections are not restricted to those seen in the spadetail mutant. Embryos are viewed from the animal pole at 40% epiboly after in situ hybridization with ntl (a, b), stat3 (c, d), or β catenin riboprobe (e, f). Embryos from spt mutant clutches exhibit gene expression patterns identical to those of wild type (a, c, e), whereas injection with 40 pg tbx16/spt dsRNA causes depletion of the message from sector-like regions of the blastoderm (b, d, f). (g) Concentration dependence of the depletion of endogenous tbx16/spt, stat3, and β catenin mRNA at 40% epiboly on the amount of injected tbx16/spt dsRNA, measured by in situ hybridization.

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Acknowledgments

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Reprinted from Developmental Biology, 224(1), Oates, A.C., Bruce, A.E., and Ho, R.K., Too much interference: injection of double-stranded RNA has nonspecific effects in the zebrafish embryo, 20-28, Copyright (2000) with permission from Elsevier. Full text @ Dev. Biol.