FIGURE

Fig. 2

ID

ZDB-FIG-080529-76

Publication

Ciruna et al., 2002 - Production of maternal-zygotic mutant zebrafish by germ-line replacement

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Fig. 2

Germ-line replacement strategy using the rhodamine-dextran labeling technique. (A) Overview of transplantation strategy showing the transfer of cells from the margin of rhodamine-dextran-labeled mutant donor embryos into the animal pole of αPGC-MO-injected WT hosts. (B) At 30 hpf, all cells within control donor embryos are efficiently labeled with rhodamine-dextran. (C) Chimeric host embryo at 30 hpf showing somatic contribution of rhodamine-dextran-labeled donor cells to anterior neuroectoderm lineages (*). Host embryos were screened for the presence of labeled donor PGCs that had migrated successfully into the gonadal mesoderm (arrow).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA