FIGURE

Fig. 7

ID

ZDB-FIG-080516-38

Publication

Qu et al., 2008 - ndrg4 is required for normal myocyte proliferation during early cardiac development in zebrafish

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Fig. 7

Attenuation of Ndrg4 induces a reduction in cell proliferation of the cardiomyocytes. Although no significant difference in the number of BrdU positive cells between control (A) and ndrg4-MO2 treated (B) embryonic hearts at 31 hpf has been detected, ndrg4-MO2 injected embryos (D) display lower number of proliferating cells than control (C) in the heart at 48 hpf. All images represent reconstructions of confocal Z-stack sections imaged on whole hearts. Green, anti-GFP antibody; Red, anti-BrdU antibody. (E) Histogram showing proliferation index (the average of the total number of BrdU positive cells in per 100 cardiomyocytes) in the heart of 48 hpf embryos: control, 13.5 ± 3.9; ndrg4-MO2, 7.8 ± 2.1 (*P < 0.01).

Expression Data

Gene:	GFP
Fish:	f1Tg f1Tg + MO2-ndrg4
Condition:	chemical treatment: 5-bromo-2'-deoxyuridine
Knockdown Reagent:	MO2-ndrg4
Anatomical Terms:	cardiac muscle cell heart heart tube
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	f1Tg + MO2-ndrg4
Condition:	chemical treatment: 5-bromo-2'-deoxyuridine
Knockdown Reagent:	MO2-ndrg4
Observed In:	cardiac muscle cell cardiac muscle cell proliferation
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 317(2), Qu, X., Jia, H., Garrity, D.M., Tompkins, K., Batts, L., Appel, B., Zhong, T.P., and Baldwin, H.S., ndrg4 is required for normal myocyte proliferation during early cardiac development in zebrafish, 486-496, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.