FIGURE

Fig. 2

ID
ZDB-FIG-080429-25
Publication
Rastegar et al., 2002 - A floor plate enhancer of the zebrafish netrin1 gene requires Cyclops (Nodal) signalling and the winged helix transcription factor FoxA2
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Screen for floor plate enhancer activity in the netrin1 locus. (A) Floor plate enhancer activity maps to the 898-bp fragment 6 located in intron 2 of the netrin1 locus. Scheme illustrating the position of fragments tested in the co-injection assay. Fragments were injected together with –1.1netrin1:lacZ, and embryos were scored for floor plate expression by β-galactosidase staining at 24 hpf. Only fragments C, 3, 4, and 6 gave significant numbers of β-galactosidase-positive cells in the floor plate (FP). (B, C) Embryos were analysed for GFP expression between 36 and 48 hpf. (B) Transient expression of the promoter –1.1netrin1:gfp. Embryos were injected with the promoter construct and analysed for GFP expression. (C) The 898-bp floor plate enhancer (FPE, fragment 6) downstream of the –1.1netrin1:gfp cassette (-1.1netrin1:gfp/FPE) directs gfp expression in the floor plate (arrow, f) and the hypochord (arrowhead), h. Expression in cells of the notochord was also noted in these transiently expressing embryos. (D, E) Transient expression of the –37Tk:gfp minimal promoter alone (D) or the 898-bp enhancer (E) inserted downstream of the thymidine kinase promoter and the gfp reporter gene (-37Tk:gfp/FPE) at 24 hpf. Insertion of the enhancer leads to expression in the floor plate (arrow) and the hypochord (arrowhead). Abbreviations: f, floor plate; h, hypochord. The scale bar represents 100 μm (B–E).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 252(1), Rastegar, S., Albert, S., Le Roux, I., Fischer, N., Blader, P., Müller, F., and Strähle, U., A floor plate enhancer of the zebrafish netrin1 gene requires Cyclops (Nodal) signalling and the winged helix transcription factor FoxA2, 1-14, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.