Fig. 3
- ID
- ZDB-FIG-080325-8
- Publication
- Sperber et al., 2008 - Zebrafish dlx2a contributes to hindbrain neural crest survival, is necessary for differentiation of sensory ganglia and functions with dlx1a in maturation of the arch cartilage elements
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dlx2a is necessary for survival of a subset of neural crest cells migrating to the pharyngeal arches. Crestin expression at 13 hpf in (A) Control (un-injected) embryo compared with, (B) an embryo injected with dlx2aMO, 0.25 mM. (B, inset) crestin expression at 15 hpf in dlx2aMO-injected embryo (0.25 mM). (C, D) Acridine orange staining of (C) control un-injected and (D) dlx2aMO-injected (0.5 mM) embryos at 14 hpf. (E, F) TUNEL-labeled transverse sections of a control (un-injected) 16 hpf embryo and an embryo injected with 0.25 mM dlx2aMO, (G, H) TUNEL labeling (black) and crestin (red) whole-mount in situ hybridization at 19 hpf. (G) Control (un-injected) embryo compared with (H) dlx2aMO injected embryo, 0.25 mM. Arrowheads in panels A and B indicate reductions of rostral crestin expression. Arrowheads in panels C, D, H indicate sites of cell death. (G, H) crestin labeled neural crest streams are labeled with Roman numerals. Anterior is to the left. cns, central nervous system; e, eye; m, mesoderm; nc, neural crest; ov otic vesicle; y, yolk. Scale bar: (A, G) 100 μm, (E) 50 μm. |
| Gene: | |
|---|---|
| Fish: | |
| Knockdown Reagent: | |
| Anatomical Term: | |
| Stage Range: | 5-9 somites to 20-25 somites |
| Fish: | |
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| Knockdown Reagent: | |
| Observed In: | |
| Stage Range: | 14-19 somites to 20-25 somites |
Reprinted from Developmental Biology, 314(1), Sperber, S.M., Saxena, V., Hatch, G., and Ekker, M., Zebrafish dlx2a contributes to hindbrain neural crest survival, is necessary for differentiation of sensory ganglia and functions with dlx1a in maturation of the arch cartilage elements, 59-70, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.