FIGURE

Fig. S4

ID

ZDB-FIG-080325-57

Publication

Cheng et al., 2008 - Zebrafish cdx1b regulates expression of downstream factors of Nodal signaling during early endoderm formation

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Fig. S4

Analyses of relationships between cdx1b and components of Nodal signaling. A slightly increased expression area of cdx1b in the intestines was detected in a 48-hpf squint^cz35 mutant embryo (n=9) (B) when compared with a sibling wild-type embryo (A). However, a subsequent semiquantitative RT-PCR revealed no significant differences when comparing cdx1b expression levels in 48-hpf squint^cz35 homozygous mutants with their sibling wild-type or AB wild-type embryos. No differences in cdx1b expression was observed in 48-hpf cyc^b16 (n=7) (D) and 4-6s oep^m134 mutants (n=26) (F) compared with sibling wild-type embryos (C,E). Respective expression levels of cyclops (n=54) and oep (n=54) in 85% epiboly cdx1b morphants (H,J) were not altered compared with wild-type embryos (G,I). Similar expression levels of sqt (n=172) and bon (n=91) were detected in 30% (L) and 40% (N) G,I). Similar expression levepiboly morphants when compared with respective wild-type embryos (K,M

Expression Data

Genes:	cdx1b mixl1 ndr1 ndr2 tdgf1
Fish:	WT ndr1^cz35/cz35 tdgf1^m134/m134 WT + MO1-cdx1b Df(Chr12:hhex,ndr2)b16/b16
Knockdown Reagent:	MO1-cdx1b
Anatomical Terms:	ectoderm endoderm gut hypoblast intestine margin mesoderm
Stage Range:	30%-epiboly to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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