ZFIN ID: ZDB-FIG-051228-6
Ebert et al., 2005 - Calcium extrusion is critical for cardiac morphogenesis and rhythm in embryonic zebrafish hearts. Proceedings of the National Academy of Sciences of the United States of America   102(49):17705-17710 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 1

Cardiac defects in homozygous tre embryos. (A and B) Cardiac development at 48 hpf. The ventricle of tre mutants (B) is small and collapsed. (C) Injection of NCX1h-morpholino (MO1) phenocopies tre.(D–F) At 72 hpf, tre mutants and morphants show pericardial edema and slightly smaller heads. Heterozygous tre embryos (not shown) have no overt phenotype. (G and H) By 48 hpf, the ventricle, denoted by ventricle-specific vmhc expression, is markedly smaller in tre mutants. (I–O) NCX1h expression by in situ hybridization. (I) NCX1h is first detected in bilateral cardiac precursors at the 12-somite stage. (J and K) At 26 hpf, NCX1h expression in the heart tube is similar to cmlc2. NCX1h expression remains restricted to the heart at (L) 32 hpf, (M) 48 hpf, (N) 72 hpf, and (O) 5 days postfertilization. A second zebrafish NCX1 gene (NCX1b) is not expressed in the heart at any stage but is expressed in brain [see Langenbacher et al. (32)]. (P) Rhythm in the atria of nine tre embryos at 48 hpf depicted graphically. Each segment represents a 1-min observation period in which the atrial rhythm was visually scored in a single mutant embryo. Wild-type sibling embryos remained in sinus rhythm throughout the entire testing period (data not shown). No reproducible patterns in rhythm are observed on day 2 (P) or 3 (not shown) or in two other experimental replicates. a, atrium; v, ventricle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myh7 WT standard conditions Long-pec cardiac ventricle ISH
slc8a1am139/m139 (AB) standard conditions Long-pec cardiac ventricle ISH
myl7 WT standard conditions Prim-5 heart tube ISH
slc8a1a WT standard conditions 10-13 somites heart primordium ISH
Prim-5 heart tube ISH
Prim-15 heart tube ISH
Long-pec heart ISH
Protruding-mouth heart ISH
Day 5 heart ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-slc8a1a standard conditions Protruding-mouth head decreased size, abnormal
Protruding-mouth pericardium edematous, abnormal
slc8a1am139/m139 (AB) standard conditions Long-pec atrium contractility, abnormal
Long-pec cardiac ventricle collapsed, abnormal
Long-pec cardiac ventricle decreased size, abnormal
Long-pec heart contraction arrhythmic, abnormal
Protruding-mouth head decreased size, abnormal
Protruding-mouth pericardium edematous, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA