FIGURE

Fig. 4

ID
ZDB-FIG-051024-3
Publication
Ghiselli et al., 2005 - D-glucuronyl C5-epimerase acts in dorso-ventral axis formation in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Effect of Glce knockdown on embryo morphogenesis. (a-d) Embryos at 1-2 cells stage were injected with a mix (8 ng each) of glce-A and glceB MO and the phenotype scored at 48 hpf. (a) wild type embryo. (b,c) mild phenotypes displaying reduced head volume and ventral fin extension. (d) severe phenotype with shortened A/P axis and loss of ventral structures. (e) Epimerase enzymatic activity in 10 hpf embryos and effect of Glce knockdown. The enzymatic assay was performed as described in Fig. 2b. (f-p) Whole mount in-situ hybridization with D/V markers of embryos at different developmental stages. (f) gata1 expression in wild-type embryos, (g) glce morphants, and (h) in embryos overexpressing glce at 24 hpf. (i-l) chordino at 50% epiboly in wild type (top) and morphants (bottom). (i,j) animal pole view, (k,l) dorsal view. (m,n) bmp2b at 70% epiboly and (o,p) at 3 somite stage in wild type (top) and morphants (bottom). Note the absence of bmp2b expression in the presumptive tail of the morphants as indicated by the arrows.

Expression Data
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Shield to Prim-5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.