Fig. 4
Restoration of endoderm rescues epibranchial ganglia in cas/sox32 mutants. (A) Diagram showing transplantation technique. Cells were grafted at blastula stages from cas mRNA-injected (or TaramA-injected, tar*), wild-type (wt) donors co-injected with biotinylated-dextran (orange) into unlabeled cas mutant hosts. Transplants gave rise to endodermal cells in the arches (box outlined in red) and induced epibranchial ganglia (blue). (B,D,E) Lateral views, 48 hpf, showing examples of epibranchial rescue by such cas-injected endodermal transplants (brown), as determined by in situ hybridization for phox2b expression (blue). pf, pectoral fin. (C) Dorsal view. Scale bar: 50 μm. |