Fig. 5

VP16-vega1 and VP16-vega2 can induce ectopic hhex expression in homozygous boz mutant embryos, and hhex expression is expanded in Df^st7 mutants. (A,B) hhex expression in a noninjected wild-type embryo. (C–F) Expression of hhex in a wild-type (C,D) and a boz mutant (E,F) embryo after injection of 100 pg VP16-vega1 mRNA. (G–J) Expression of hhex in a wild-type (G,H) and a boz (I, J) embryo after injection of 100 pg VP16-vega2 mRNA. (K,L) Expression of hhex in a noninjected embryo (K) and in an embryo injected with 25 pg of vega2 mRNA (L). (M,N) Expression of boz in a noninjected embryo (M) and in an embryo injected with 100 pg VP16-vega2 mRNA (N). (O–R) hhex expression in wild-type and heterozygous Df^st7 embryos (O,P) and in a Df^st7 homozygous mutant embryo (Q–R). Pictures are either focussed to the midline (O,Q) or the lateral marginal region (P,R). All embryos were fixed at 50% epiboly stage. All embryos depicted in A–J and O–R were genotyped after photography. (A,C,E,G,I,K–N: animal views; B,D,F,H,J,O–R: lateral views; A,B,K,M,O,P: dorsal to the right, all others, except for L,Q,R, putative dorsal to the right; probes used are indicated in the bottom left corner of each panel).