CRISPR

CRISPR1-hmgcs1

ID

ZDB-CRISPR-250822-3

Name

CRISPR1-hmgcs1

Previous Names

None

Target

hmgcs1 (1)

Sequence

5' - GCTTGGTCAACGTACTGAGA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
mnu15	hmgcs1

Expression

Gene expression in Wild Types + CRISPR1-hmgcs1

No data available

Phenotype

Phenotype resulting from CRISPR1-hmgcs1

No data available

Phenotype of all Fish created by or utilizing CRISPR1-hmgcs1

Phenotype	Fish	Conditions	Figures
whole organism decreased pigmentation, abnormal	hmgcs1^mnu15/mnu15	standard conditions	Fig. 6 from Dofash et al., 2024
whole organism mobility, ameliorated	hmgcs1^mnu15/mnu15	chemical treatment by environment: mevalonic acid	Fig. 7 from Dofash et al., 2024
skeletal muscle mitochondrion structure, abnormal	hmgcs1^mnu15/mnu15	standard conditions	Fig. 6 from Dofash et al., 2024
skeletal muscle mitochondrial inner membrane morphology, ameliorated	hmgcs1^mnu15/mnu15	chemical treatment by environment: mevalonic acid	Fig. 7 from Dofash et al., 2024
skeletal muscle mitochondrial inner membrane morphology, abnormal	hmgcs1^mnu15/mnu15	standard conditions	Fig. 6, Fig. 7 from Dofash et al., 2024
thigmotaxis absent process, abnormal	hmgcs1^mnu15/mnu15	standard conditions	Fig. 6 from Dofash et al., 2024
whole organism immobile, abnormal	hmgcs1^mnu15/mnu15	standard conditions	Fig. 6 from Dofash et al., 2024
whole organism edematous, abnormal	hmgcs1^mnu15/mnu15	chemical treatment by environment: mevalonic acid	Fig. 7 from Dofash et al., 2024
whole organism viability, ameliorated	hmgcs1^mnu15/mnu15	chemical treatment by environment: mevalonic acid	Fig. 7 from Dofash et al., 2024
head deformed, abnormal	hmgcs1^mnu15/mnu15	chemical treatment by environment: mevalonic acid	Fig. 7 from Dofash et al., 2024
skeletal muscle mitochondrial inner membrane invaginated, abnormal	hmgcs1^mnu15/mnu15	standard conditions	Fig. 6 from Dofash et al., 2024
brain accumulation blood, abnormal	hmgcs1^mnu15/mnu15	standard conditions	Fig. 6 from Dofash et al., 2024
whole organism dead, abnormal	hmgcs1^mnu15/mnu15	standard conditions	Fig. 6 from Dofash et al., 2024

Citations