CRISPR

CRISPR6-myh9b

ID

ZDB-CRISPR-250429-15

Name

CRISPR6-myh9b

Previous Names

None

Target

myh9b (1)

Sequence

5' - GGGCCGGGACTACGTGCAGA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
mke414	myh9b

Expression

Gene expression in Wild Types + CRISPR6-myh9b

No data available

Phenotype

Phenotype resulting from CRISPR6-myh9b

No data available

Phenotype of all Fish created by or utilizing CRISPR6-myh9b

Phenotype	Fish	Conditions	Figures
whole organism myh9a expression decreased amount, abnormal	myh9b^{mke414/mke414}	standard conditions	Fig. 5. , Fig. 6. from Rolfs et al., 2024
whole organism viability, abnormal	myh9b^{mke414/mke414}	standard conditions	Fig. 4. from Rolfs et al., 2024
pericardium edematous, abnormal	myh9b^{mke414/mke414}	standard conditions	Fig. 3. from Rolfs et al., 2024
whole organism myh9b expression decreased amount, abnormal	myh9b^{mke414/mke414}	standard conditions	Fig. 5. from Rolfs et al., 2024
whole organism myh9a expression decreased amount, abnormal	myh9b^mke414/+	standard conditions	Fig. 5. , Fig. 6. from Rolfs et al., 2024
whole organism myh9b expression decreased amount, abnormal	myh9b^mke414/+	standard conditions	Fig. 5. from Rolfs et al., 2024
whole organism myh10 expression decreased amount, abnormal	myh9b^mke414/+	standard conditions	Fig. 5. from Rolfs et al., 2024
extension decreased length, abnormal	myh9a^{sa20632/sa20632}; myh9b^{mke414/mke414}	standard conditions	Fig. 7. from Rolfs et al., 2024
pericardium edematous, abnormal	myh9a^{sa20632/sa20632}; myh9b^{mke414/mke414}	standard conditions	Fig. 7. from Rolfs et al., 2024
yolk edematous, abnormal	myh9a^{sa20632/sa20632}; myh9b^{mke414/mke414}	standard conditions	Fig. 7. from Rolfs et al., 2024
whole organism cystic, abnormal	myh9a^{sa20632/sa20632}; myh9b^{mke414/mke414}	standard conditions	Fig. 7. from Rolfs et al., 2024
integument blistered, abnormal	myh9a^{sa20632/sa20632}; myh9b^{mke414/mke414}	standard conditions	Fig. 7. from Rolfs et al., 2024
pericardium edematous, abnormal	myh10^{mke508/mke508}; myh9b^{mke414/mke414}	standard conditions	Fig. 7. from Rolfs et al., 2024
yolk edematous, abnormal	myh10^{mke508/mke508}; myh9b^{mke414/mke414}	standard conditions	Fig. 7. from Rolfs et al., 2024
whole organism cystic, abnormal	myh10^{mke508/mke508}; myh9b^{mke414/mke414}	standard conditions	Fig. 7. from Rolfs et al., 2024
axis curved, abnormal	myh10^{mke508/mke508}; myh9b^{mke414/mke414}	standard conditions	Fig. 7. from Rolfs et al., 2024
male organism male sterile, abnormal	myh10^{mke508/mke508}; myh9b^{mke414/mke414}	standard conditions	Fig. 7. from Rolfs et al., 2024
multicellular organism development arrested, abnormal	myh10^{mke508/mke508}; myh9b^{mke414/mke414}	standard conditions	Fig. 7. from Rolfs et al., 2024

Citations