CRISPR

CRISPR1-uba2

ID
ZDB-CRISPR-230613-6
Name
CRISPR1-uba2
Previous Names
None
Target
Sequence
5' - GAGCTCCTGAAAAACC - 3'
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "TGG" at the 3' end.
Genome Resources
None
Target Location
View all 4 target locations
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
nni101 uba2
nni102 uba2
Expression
Gene expression in Wild Types + CRISPR1-uba2
No data available
Phenotype
Phenotype resulting from CRISPR1-uba2
No data available
Phenotype of all Fish created by or utilizing CRISPR1-uba2
Phenotype Fish Conditions Figures
heart contraction decreased process quality, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 3: with image from Schnur et al., 2021
brain neuron decreased amount, abnormal uba2nni101/nni101 (AB/TL) standard conditions Fig. S2 from Schnur et al., 2021
midbrain decreased size, abnormal uba2nni101/nni101 (AB/TL) standard conditions Fig. S2 from Schnur et al., 2021
pectoral fin cartilage morphology, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 4: with image from Schnur et al., 2021
eye decreased size, abnormal uba2nni101/nni101 (AB/TL) standard conditions Fig. S2Figure 3: with image from Schnur et al., 2021
ventricular system swollen, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 3: with image from Schnur et al., 2021
pericardium edematous, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 3: with imageFigure 4: with image from Schnur et al., 2021
head decreased size, abnormal uba2nni101/nni101 (AB/TL) standard conditions Fig. S2Figure 4: with image from Schnur et al., 2021
whole organism dead, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 3: with image from Schnur et al., 2021
ceratobranchial cartilage morphology, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 4: with image from Schnur et al., 2021
swim bladder uninflated, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 3: with imageFigure 4: with image from Schnur et al., 2021
lens morphology, abnormal uba2nni101/nni101 (AB/TL) standard conditions Fig. S2 from Schnur et al., 2021
pectoral fin decreased length, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 3: with image from Schnur et al., 2021
median fin fold ab1-col2a labeling spatial pattern, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 4: with image from Schnur et al., 2021
head edematous, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 3: with imageFigure 4: with image from Schnur et al., 2021
post-vent region curved ventral, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 3: with image from Schnur et al., 2021
caudal fin rough, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 3: with image from Schnur et al., 2021
Meckel's cartilage morphology, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 4: with image from Schnur et al., 2021
pectoral fin oblique orientation, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 3: with image from Schnur et al., 2021
retina decreased thickness, abnormal uba2nni101/nni101 (AB/TL) standard conditions Fig. S2 from Schnur et al., 2021
eye photoreceptor cell zpr-1 labeling decreased distribution, abnormal uba2nni101/nni101 (AB/TL) standard conditions Fig. S2 from Schnur et al., 2021
caudal fin broken, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 3: with image from Schnur et al., 2021
brain grey matter decreased ratio brain white matter, abnormal uba2nni101/nni101 (AB/TL) standard conditions Fig. S2 from Schnur et al., 2021
ceratohyal cartilage morphology, abnormal uba2nni101/nni101 (AB/TL) standard conditions Figure 4: with image from Schnur et al., 2021
whole organism viable, abnormal uba2nni101/+ (AB/TL) standard conditions Figure 3: with image from Schnur et al., 2021
Citations