CRISPR

CRISPR1-uba2

ID

ZDB-CRISPR-230613-6

Name

CRISPR1-uba2

Previous Names

None

Target

uba2 (1)

Sequence

5' - GAGCTCCTGAAAAACC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

View all 4 target locations

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
nni101	uba2
nni102	uba2

Expression

Gene expression in Wild Types + CRISPR1-uba2

No data available

Phenotype

Phenotype resulting from CRISPR1-uba2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-uba2

Phenotype	Fish	Conditions	Figures
heart contraction decreased process quality, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Figure 3: from Schnur et al., 2021
brain neuron decreased amount, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Fig. S2 from Schnur et al., 2021
midbrain decreased size, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Fig. S2 from Schnur et al., 2021
pectoral fin cartilage morphology, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Figure 4: from Schnur et al., 2021
eye decreased size, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Fig. S2, Figure 3: from Schnur et al., 2021
ventricular system swollen, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Figure 3: from Schnur et al., 2021
pericardium edematous, abnormal	uba2^{nni101/nni101} (AB/TL)	control	Figure 3: , Figure 4: from Schnur et al., 2021
head decreased size, abnormal	uba2^{nni101/nni101} (AB/TL)	control	Fig. S2, Figure 4: from Schnur et al., 2021
whole organism dead, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Figure 3: from Schnur et al., 2021
ceratobranchial cartilage morphology, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Figure 4: from Schnur et al., 2021
swim bladder uninflated, abnormal	uba2^{nni101/nni101} (AB/TL)	control	Figure 3: , Figure 4: from Schnur et al., 2021
lens morphology, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Fig. S2 from Schnur et al., 2021
pectoral fin decreased length, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Figure 3: from Schnur et al., 2021
median fin fold ab1-col2a labeling spatial pattern, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Figure 4: from Schnur et al., 2021
head edematous, abnormal	uba2^{nni101/nni101} (AB/TL)	control	Figure 3: , Figure 4: from Schnur et al., 2021
post-vent region curved ventral, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Figure 3: from Schnur et al., 2021
caudal fin rough, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Figure 3: from Schnur et al., 2021
Meckel's cartilage morphology, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Figure 4: from Schnur et al., 2021
pectoral fin oblique orientation, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Figure 3: from Schnur et al., 2021
retina decreased thickness, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Fig. S2 from Schnur et al., 2021
eye photoreceptor cell zpr-1 labeling decreased distribution, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Fig. S2 from Schnur et al., 2021
caudal fin broken, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Figure 3: from Schnur et al., 2021
brain grey matter decreased ratio brain white matter, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Fig. S2 from Schnur et al., 2021
ceratohyal cartilage morphology, abnormal	uba2^{nni101/nni101} (AB/TL)	standard conditions	Figure 4: from Schnur et al., 2021
whole organism viable, abnormal	uba2^nni101/+ (AB/TL)	standard conditions	Figure 3: from Schnur et al., 2021

Citations