CRISPR

CRISPR1-ap1g1

ID

ZDB-CRISPR-230130-1

Name

CRISPR1-ap1g1

Previous Names

None

Target

ap1g1 (1)

Sequence

5' - GACGGCTCGAACCCAGGCAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ubx2	ap1g1

Expression

Gene expression in Wild Types + CRISPR1-ap1g1

No data available

Phenotype

Phenotype resulting from CRISPR1-ap1g1

No data available

Phenotype of all Fish created by or utilizing CRISPR1-ap1g1

Phenotype	Fish	Conditions	Figures
whole organism dead, abnormal	ap1g1^ubx2/ubx2 (AB)	standard conditions	Figure 1 , Figure 2 from Mignani et al., 2023
whole organism ap1g1 expression decreased amount, abnormal	ap1g1^ubx2/ubx2 (AB)	standard conditions	Figure 1 from Mignani et al., 2023
blastomere morphology, abnormal	ap1g1^ubx2/ubx2 (AB)	standard conditions	Figure 1 from Mignani et al., 2023
whole organism bmp7a expression decreased amount, abnormal	ap1g1^ubx2/+ (AB)	standard conditions	Figure 7 from Mignani et al., 2023
intestinal epithelium detached from intestinal lamina propria mucosa, abnormal	ap1g1^ubx2/+ (AB)	standard conditions	Figure 4 from Mignani et al., 2023
male organism decreased male fertility, abnormal	ap1g1^ubx2/+ (AB)	standard conditions	Figure 3 from Mignani et al., 2023
intestine columnar/cuboidal epithelial cell morphology, abnormal	ap1g1^ubx2/+ (AB)	standard conditions	Figure 4 from Mignani et al., 2023
intestinal lamina propria mucosa disorganized, abnormal	ap1g1^ubx2/+ (AB)	standard conditions	Figure 4 from Mignani et al., 2023
whole organism bmp2b expression decreased amount, abnormal	ap1g1^ubx2/+ (AB)	standard conditions	Figure 7 from Mignani et al., 2023
ovary disorganized, abnormal	ap1g1^ubx2/+ (AB)	standard conditions	Figure 3 from Mignani et al., 2023
testis ap1g1 expression decreased amount, abnormal	ap1g1^ubx2/+ (AB)	standard conditions	Figure 3 from Mignani et al., 2023
whole organism decreased weight, abnormal	ap1g1^ubx2/+ (AB)	standard conditions	Figure 4 from Mignani et al., 2023
whole organism bmp4 expression decreased amount, abnormal	ap1g1^ubx2/+ (AB)	standard conditions	Figure 7 from Mignani et al., 2023
ovary ap1g1 expression decreased amount, abnormal	ap1g1^ubx2/+ (AB)	standard conditions	Figure 3 from Mignani et al., 2023
whole organism cdh1 expression decreased amount, abnormal	ap1g1^ubx2/+ (AB)	standard conditions	Figure 7 from Mignani et al., 2023
intestine lumen goblet cell morphology, abnormal	ap1g1^ubx2/+ (AB)	standard conditions	Figure 4 from Mignani et al., 2023
oocyte degenerate, abnormal	ap1g1^ubx2/+ (AB)	standard conditions	Figure 3 from Mignani et al., 2023
midbrain EGFP expression decreased distribution, abnormal	ap1g1^ubx2/+; nl1Tg (AB)	standard conditions	Figure 5 from Mignani et al., 2023
spinal cord EGFP expression decreased distribution, abnormal	ap1g1^ubx2/+; nl1Tg (AB)	standard conditions	Figure 5 from Mignani et al., 2023
telencephalon neuron decreased amount, abnormal	ap1g1^ubx2/+; nl1Tg (AB)	standard conditions	Figure 5 from Mignani et al., 2023
telencephalon anterior region EGFP expression decreased distribution, abnormal	ap1g1^ubx2/+; nl1Tg (AB)	standard conditions	Figure 5 from Mignani et al., 2023
brain decreased volume, abnormal	ap1g1^ubx2/+; nl1Tg (AB)	standard conditions	Figure 6 from Mignani et al., 2023
midbrain hindbrain boundary neuron decreased amount, abnormal	ap1g1^ubx2/+; nl1Tg (AB)	standard conditions	Figure 5 from Mignani et al., 2023
midbrain hindbrain boundary EGFP expression decreased distribution, abnormal	ap1g1^ubx2/+; nl1Tg (AB)	standard conditions	Figure 5 from Mignani et al., 2023
spinal cord neuron decreased amount, abnormal	ap1g1^ubx2/+; nl1Tg (AB)	standard conditions	Figure 5 from Mignani et al., 2023
midbrain neuron decreased amount, abnormal	ap1g1^ubx2/+; nl1Tg (AB)	standard conditions	Figure 5 from Mignani et al., 2023

Citations