CRISPR

CRISPR3-vtg

ID

ZDB-CRISPR-200812-6

Name

CRISPR3-vtg

Previous Names

sg13_Fw (1)

Targets

vtg1 (1)
vtg2 (1)
vtg4 (1)
vtg5 (1)
vtg6 (1)
vtg7 (1)

Sequence

5' - GGCTCTTAAAGCTTTGCTTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGA" at the 5' end.

Genome Resources

None

Target Location

View all 10 target locations

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
inr101	vtg1

Expression

Gene expression in Wild Types + CRISPR3-vtg

No data available

Phenotype

Phenotype resulting from CRISPR3-vtg

No data available

Phenotype of all Fish created by or utilizing CRISPR3-vtg

Phenotype	Fish	Conditions	Figures
yolk edematous, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 9 from Yilmaz et al., 2019
female organism unfertilized egg vtg5 expression absent, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 5 from Yilmaz et al., 2019
female organism liver vtg7 expression increased amount, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 4, Fig. 5 from Yilmaz et al., 2019
female organism unfertilized egg vtg1 expression absent, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 5 from Yilmaz et al., 2019
fertilized egg hatching decreased rate of occurrence, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 7 from Yilmaz et al., 2019
female organism liver vtg5 expression absent, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 4, Fig. 5 from Yilmaz et al., 2019
whole organism decreased life span, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 8 from Yilmaz et al., 2019
female organism unfertilized egg vtg7 expression increased amount, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 5 from Yilmaz et al., 2019
post-vent region bent, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 9 from Yilmaz et al., 2019
female organism unfertilized egg vtg4 expression absent, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 5 from Yilmaz et al., 2019
post-vent region curved, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 9 from Yilmaz et al., 2019
whole organism dead, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 8 from Yilmaz et al., 2019
female organism unfertilized egg vtg3 expression increased amount, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 5 from Yilmaz et al., 2019
pericardium edematous, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 9 from Yilmaz et al., 2019
female organism oocyte increased amount, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 7 from Yilmaz et al., 2019
female organism liver vtg1 expression absent, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 4, Fig. 5 from Yilmaz et al., 2019
female organism liver vtg4 expression absent, abnormal	vtg1^{inr101/inr101} (TU)	standard conditions	Fig. 4, Fig. 5 from Yilmaz et al., 2019
female organism liver vtg4 expression decreased amount, abnormal	vtg1^inr101/+ (TU)	standard conditions	Fig. 4 from Yilmaz et al., 2019
female organism liver vtg1 expression decreased amount, abnormal	vtg1^inr101/+ (TU)	standard conditions	Fig. 4 from Yilmaz et al., 2019

Citations