CRISPR

CRISPR1-park7

ID

ZDB-CRISPR-200429-13

Name

CRISPR1-park7

Previous Names

None

Target

park7 (1)

Sequence

5' - GGTGGATGTGATGCGCAGAG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was CGG at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ub2000	park7

Expression

Gene expression in Wild Types + CRISPR1-park7

No data available

Phenotype

Phenotype resulting from CRISPR1-park7

No data available

Phenotype of all Fish created by or utilizing CRISPR1-park7

Phenotype	Fish	Conditions	Figures
brain Ab3-park7 labeling absent, abnormal	park7^{ub2000/ub2000}	standard conditions	Figure 1 from Gharbi et al., 2021
retinal pigmented epithelium composition, abnormal	park7^{ub2000/ub2000}	standard conditions	Figure 3 , Figure 4 from Gharbi et al., 2021
whole organism decreased weight, abnormal	park7^{ub2000/ub2000}	standard conditions	Fig. 2 from Edson et al., 2019
whole organism park7 expression absent, abnormal	park7^{ub2000/ub2000}	standard conditions	Fig. 1 from Edson et al., 2019
brain park7 expression absent, abnormal	park7^{ub2000/ub2000}	standard conditions	Fig. 4 from Edson et al., 2019
retinal ganglion cell decreased amount, abnormal	park7^{ub2000/ub2000}	standard conditions	Figure 2 from Gharbi et al., 2021
retinal pigmented epithelium phagocytic vesicle increased amount, abnormal	park7^{ub2000/ub2000}	standard conditions	Figure 4 from Gharbi et al., 2021
brain AB1-abce1 labeling increased amount, abnormal	park7^{ub2000/ub2000}	standard conditions	Fig. 4 from Edson et al., 2019
brain AB1-gsta labeling increased amount, abnormal	park7^{ub2000/ub2000}	standard conditions	Fig. 4 from Edson et al., 2019
retinal photoreceptor layer organization quality, abnormal	park7^{ub2000/ub2000}	standard conditions	Figure 2 from Gharbi et al., 2021
NADH dehydrogenase (ubiquinone) activity decreased efficacy, abnormal	park7^{ub2000/ub2000}	standard conditions	Fig. 2 from Edson et al., 2019
retina decreased thickness, abnormal	park7^{ub2000/ub2000}	standard conditions	Figure 2 from Gharbi et al., 2021
Muller cell Ab19-gfp labeling absent, abnormal	park7^{ub2000/ub2000}	standard conditions	Figure 1 from Gharbi et al., 2021
retinal pigmented epithelium vacuole increased amount, abnormal	park7^{ub2000/ub2000}	standard conditions	Figure 2 , Figure 3 , Figure 4 from Gharbi et al., 2021
brain ab1-th labeling decreased amount, abnormal	park7^{ub2000/ub2000}	standard conditions	Fig. 2 from Edson et al., 2019
slow muscle cell mitochondrion morphology, abnormal	park7^{ub2000/ub2000} (AB/TU)	standard conditions	Figure 2 from Rostad et al., 2024
slow muscle cell mitochondrion degenerate, abnormal	park7^{ub2000/ub2000} (AB/TU)	standard conditions	Figure 2 from Rostad et al., 2024
male organism slow muscle cell decreased amount, abnormal	park7^{ub2000/ub2000} (AB/TU)	standard conditions	Figure 1 from Rostad et al., 2024
male organism fast muscle cell decreased amount, abnormal	park7^{ub2000/ub2000} (AB/TU)	standard conditions	Figure 1 from Rostad et al., 2024
slow muscle cell autophagy increased process quality, abnormal	park7^{ub2000/ub2000} (AB/TU)	standard conditions	Figure 2 from Rostad et al., 2024
slow muscle cell increased distance slow muscle cell, abnormal	park7^{ub2000/ub2000} (AB/TU)	standard conditions	Figure 1 from Rostad et al., 2024
muscle NAD+ biosynthetic process decreased process quality, abnormal	park7^{ub2000/ub2000} (AB/TU)	standard conditions	Figure 5 from Rostad et al., 2024
male organism skeletal muscle cell atrophied, abnormal	park7^{ub2000/ub2000} (AB/TU)	standard conditions	Figure 1 from Rostad et al., 2024
brain Ab3-park7 labeling amount, ameliorated	park7^{ub2000/ub2000}; ub1001Tg	standard conditions	Figure 1 from Gharbi et al., 2021
retinal pigmented epithelium vacuole amount, ameliorated	park7^{ub2000/ub2000}; ub1001Tg	standard conditions	Figure 2 , Figure 3 from Gharbi et al., 2021
retinal photoreceptor layer organization quality, ameliorated	park7^{ub2000/ub2000}; ub1001Tg	standard conditions	Figure 2 from Gharbi et al., 2021
retinal pigmented epithelium composition, ameliorated	park7^{ub2000/ub2000}; ub1001Tg	standard conditions	Figure 3 from Gharbi et al., 2021
retinal ganglion cell amount, ameliorated	park7^{ub2000/ub2000}; ub1001Tg	standard conditions	Figure 2 from Gharbi et al., 2021
retina thickness, ameliorated	park7^{ub2000/ub2000}; ub1001Tg	standard conditions	Figure 2 from Gharbi et al., 2021
brain Ab3-park7 labeling amount, ameliorated	park7^{ub2000/ub2000}; ub2003Tg	standard conditions	Figure 1 from Gharbi et al., 2021
retinal photoreceptor layer organization quality, ameliorated	park7^{ub2000/ub2000}; ub2003Tg	standard conditions	Figure 2 from Gharbi et al., 2021
retinal pigmented epithelium composition, ameliorated	park7^{ub2000/ub2000}; ub2003Tg	standard conditions	Figure 3 from Gharbi et al., 2021
retinal ganglion cell decreased amount, abnormal	park7^{ub2000/ub2000}; ub2003Tg	standard conditions	Figure 2 from Gharbi et al., 2021
retinal pigmented epithelium vacuole increased amount, abnormal	park7^{ub2000/ub2000}; ub2003Tg	standard conditions	Figure 2 , Figure 3 from Gharbi et al., 2021
retina thickness, ameliorated	park7^{ub2000/ub2000}; ub2003Tg	standard conditions	Figure 2 from Gharbi et al., 2021

Citations