Genomic Feature
fh227
- ID
- ZDB-ALT-061129-2
- Name
- fh227
- Synonyms
- None
- Affected Genomic Region
- Construct
- None
- Type
- Allele with one point mutation
- Protocol
- adult males treated with ENU
- Lab of Origin
- Moens Lab
- Current Source
- Zebrafish International Resource Center (ZIRC) ( order this )
- Other Pages
Notes
No data available
Variants
- Variant Type
- Point Mutation
- Variant Location
- Chr 12: 8484727 (GRCz11) (1) Details
- Nucleotide change
- G/T
- Variant Notes
Effect on DNA/cDNA, transcript, protein (from publications)
- DNA/cDNA Change
- C>A at position 1002 (1)
- Transcript Consequence
- Premature Stop (1)
- Protein Consequence
- Cys>Stop at position 334 (1)
- Flanking Sequence
-
TATCTAAGGTTATTTTATTATTATTATTAAATTAATTGGCCTAAATATTCAAACAAAGTGCGTTTGGGTCAGTCAGCATGCGACTGAGGCATTTAAAAACAACTTTGCATCACCTTTACTAAAAGTGTGCGCAAAGAAAGTGTCCATGTTTGGCACTGTGGTTTGATTTCACCTCATCAGTCCACCCAGTTTTAATAAAACACGAACGGTGCATTATTGTATTGCCTACATAGTGTTGGATATTGCACGGTCCAGTTGAAAGTTCACTGGTTTGAACTGGACGAGCAGATGCTCGTGGCGACGCCGCGCTCTGAGCTGGACACTCCTGTGGACGACGAGGAGGACTTTCGCTCTTTTTGTCGCAGGTGGATTTTGGTGTGTCTCTTTCTTTCGTCGCTCCTCGCGAACTTTCTCCCGCAAAAGTCGCAGGCGAACGGCTTTTCGCCGGTGTGCGTGCGAATGTGGGTCGTCAGGTGGTCGCTGCGACTAAAGTTCCTCAT
G/T CATATCCGACACTGGAAGGGTTTGTGGCCGGTGTGGATGCGGATGTGTCTGGTAAGTTCGTCCGAGCGCGAGAAGCGCCTGTCGCAGCCCTCCGCCGGACAGGGGTAGGGCCGCTCGTGCACGGGCGTCTTGCTGGGTCTGTTGGGGTATTTCCTTGGACGCAGGATTGGCCTGAGGGGCAAATTCTGCGGAGTGTAAGCTGTGGGTAGCCGGGGTCCGTCAGGCACTGGTCCCCCTAGCGTGAAGTTCCTGATAGTGTTCAGGGGGGTTAGAGGAGGCGGTAATTTGAAAGAGTCCAGCGGGCAGGAGAACGGTTTGCGGTCGGGCATCGACTGCATGTCCCTCTGGCACGGAGGTTGGAAAAAGCCGGCGTAGTCCGGGATTATAGGGAACAACCCAGAGTCGGCGTTTGGCTTTGGAGAGGAGTAGGATGGCGGAGGATATGAGATGGGACAGGTGGAGGTGGAAAGGAACGCAGACGGGTCTTGATAGACCTCTCC - Additional Sequence
- None
Fish
| Fish | Genomic Feature Zygosity | Parental Zygosity | Affected Genomic Regions | Phenotype | Gene Expression |
|---|---|---|---|---|---|
| egr2bfh227/fh227 | Homozygous | Unknown | Fig. 1 from Torbey et al., 2018 | ||
| egr2bfh227/fh227 | Homozygous | ♀+/- ♂+/- | 3 figures  from 2 publications | 5 figures from 5 publications | |
| egr2bfh227/fh227 (AB) | Homozygous | ♀+/- ♂+/- | text only from Miller et al., 2013 | ||
| egr2bfh227/+ (AB) | Heterozygous | Unknown | |||
| egr2bfh227/fh227; zf2225Tg | Complex | 2 figures from Torbey et al., 2018 | |||
| egr2bfh227/fh227; zf2227Tg | Complex | Fig. 5 from Torbey et al., 2018 | |||
| egr2bfh227/fh227; zf2228Tg | Complex | Fig. 5 from Torbey et al., 2018 | |||
| egr2bfh227/fh227; zf2229Tg | Complex | Fig. 5 from Torbey et al., 2018 | |||
| egr2bfh227/fh227; zf2231Tg | Complex | Fig. 7 from Torbey et al., 2018 | |||
| egr2bfh227/fh227; zf2232Tg | Complex | Fig. 7 from Torbey et al., 2018 |
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Supplemental Information
- Genotyping protocol
- fh227.pdf
- Torbey, P., Thierion, E., Collombet, S., de Cian, A., Desmarquet-Trin-Dinh, C., Dura, M., Concordet, J.P., Charnay, P., Gilardi-Hebenstreit, P. (2018) Cooperation, cis-interactions, versatility and evolutionary plasticity of multiple cis-acting elements underlie krox20 hindbrain regulation. PLoS Genetics. 14:e1007581
- Sanchez, N.E., Harty, B.L., O'Reilly-Pol, T., Ackerman, S.D., Herbert, A.L., Holmgren, M., Johnson, S.L., Gray, R.S., Monk, K.R. (2017) Whole Genome Sequencing-Based Mapping and Candidate Identification of Mutations from Fixed Zebrafish Tissue. G3 (Bethesda). 7(10):3415-3425
- Labalette, C., Wassef, M.A., Desmarquet-Trin Dinh, C., Bouchoucha, Y.X., Le Men, J., Charnay, P., Gilardi-Hebenstreit, P. (2015) Molecular dissection of segment formation in the developing hindbrain. Development (Cambridge, England). 142:185-95
- Zigman, M., Laumann-Lipp, N., Titus, T., Postlethwait, J., and Moens, C.B. (2014) Hoxb1b controls oriented cell division, cell shape and microtubule dynamics in neural tube morphogenesis. Development (Cambridge, England). 141(3):639-649
- Bouchoucha, Y.X., Reingruber, J., Labalette, C., Wassef, M.A., Thierion, E., Desmarquet-Trin Dinh, C., Holcman, D., Gilardi-Hebenstreit, P., and Charnay, P. (2013) Dissection of a Krox20 positive feedback loop driving cell fate choices in hindbrain patterning. Molecular Systems Biology. 9:690
- Miller, A.C., Obholzer, N.D., Shah, A.N., Megason, S.G., and Moens, C.B. (2013) RNA-seq based mapping and candidate identification of mutations from forward genetic screens. Genome research. 23(4):679-686
- Labalette, C., Bouchoucha, Y.X., Wassef, M.A., Gongal, P.A., Le Men, J., Becker, T., Gilardi-Hebenstreit, P., and Charnay, P. (2011) Hindbrain patterning requires fine-tuning of early krox20 transcription by Sprouty 4. Development (Cambridge, England). 138(2):317-326
- Monk, K.R., Naylor, S.G., Glenn, T.D., Mercurio, S., Perlin, J.R., Dominguez, C., Moens, C.B., and Talbot, W.S. (2009) A G protein-coupled receptor is essential for Schwann cells to initiate myelination. Science (New York, N.Y.). 325(5946):1402-1405
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