FIGURE SUMMARY
Title

Loss of Panx1 function in zebrafish alters motor behavior in a lab-on-chip model of Parkinson's disease

Authors
Khalili, A., Safarian, N., van Wijngaarden, E., Zoidl, G.S., Zoidl, G.R., Rezai, P.
Source
Full text @ J. Neurosci. Res.
Fig. 1

Microfluidic device and experimental setup for screening the electric-induced response of semi-immobilized 5-7dpf zebrafish larva. (a) The experimental setup made up of a microscope, two syringe pumps, a sourcemeter, computer, camera, and the microfluidic device connected via tubing and wires. (b) The device consists of a sloped inlet, rounded side channels, a main channel, trapping region (TR), screening pool, outlet, two electrodes, and valve channel. (c) Close-up view of the TR and screening pool showing a semi-immobilized 5dpf larva.

Fig. 2

Electric-induced (a) RD and (b) TBF of 5dpf panx1a+/+ and panx1a−/− zebrafish larvae exposed to 250 μM 6-OHDA for 0 and 72 h. (15 larvae per experimental condition in three independent trials, N = 45). All data were compared with the panx1a+/+ control group. **p < .01, ***p < .001.

Fig. 3

Differential expression of genes of the dopaminergic pathway in the 6-OHDA model at 5dpf. The bar charts show the relative expression ratios of nine genes quantified by RT-qPCR. We compared the expression of 6-OHDA-treated larvae to untreated siblings for both genotypes. Green bars represent statistically significant upregulations (p < .05). Statistical test: Relative Expression Software Tool (REST-2009) (Pfaffl, 2002). The number of experimental replicates (n = 3).

Fig. 4

Electric-induced (a) RD and (b) TBF of 7dpf panx1a+/+ and panx1a−/− zebrafish larvae exposed to 250 μM 6-OHDA for 0, 72 and 120 h (15 larvae per experimental condition in three independent trials, N = 45). All data were compared with the panx1a+/+ control group. *p < .05, **p < .01, ***p < .001.

Fig. 5

Differential expression of genes of the dopaminergic pathway in the 6-OHDA model at 7dpf. The bar charts show the relative expression ratios of nine genes quantified by RT-qPCR. We compared the expression of 6-OHDA-treated larvae to untreated siblings for both genotypes. The green bar represents a statistically significant upregulation (p < .05). Statistical test: Relative Expression Software Tool (REST-2009) (Pfaffl, 2002). Number of experimental replicates (n = 3).

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Neurosci. Res.
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