- Title
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Novel Development of Magnetic Resonance Imaging to Quantify the Structural Anatomic Growth of Diverse Organs in Adult and Mutant Zebrafish
- Authors
- Sharma, S., Magnitsky, S., Reesey, E., Schwartz, M., Haroon, S., Lavorato, M., Chan, S., Xiao, R., Wilkins, B.J., Martinez, D., Seiler, C., Falk, M.J.
- Source
- Full text @ Zebrafish
MRIs of 9-month-old WT zebrafish with (1A) sagittal, (1B) axial, (1C) coronal sections, and (1D) 3D reconstruction. Organs analyzed include (a) brain, (b) kidneys, (c) liver, (d) spinal cord, (e) heart, (f) swim bladder, (g) ears/otolith organ, (h) eyes, and (i) optic nerve. 3D, three-dimensional; MRI, magnetic resonance imaging; WT, wild type. |
Three sagittal histologic sections of 9-month-old WT zebrafish, with annotation of selected tissues. |
Comparative volumetric analysis from 5 to 31 months post-fertilization of organ volumes in adult WT zebrafish. Each dot indicates a single animal. |
Comparative volumetric analysis in surf1−/− mutants from 5 to 19 months post-fertilization relative to age-matched WT controls. Each dot indicates a single animal. Black line and dots are WT, gray line and triangles are surf1−/−. |
Comparison of organ volumes of three mitochondrial disease mutants (triangles) versus age-matched WT adult zebrafish (circles). From left to right, 5-month-old WT, 5-month-old surf1−/− mutants; 12–14-month-old WT, 12–14-month-old surf1−/− mutants; 16–18-month-old WT, 16–18-month-old opa1+/− mutants, 19-month-old surf1−/− mutants; 31-month-old WT, 31-month-old fbxl4−/− mutant zebrafish. #0.10 < p > 0.05, *p < 0.05, **p < 0.01. |
. Comparison of animal weight in three mitochondrial disease mutants (triangles) versus age-matched WT (circles) adult zebrafish. From left to right, 5-month-old WT, 5-month-old surf1−/− mutants; 12–14-month-old WT, 12–14-month-old surf1−/− mutants; 16–18-month-old WT, 16–18-month-old opa1+/− mutants, 19-month-old surf1−/− mutants; 31-month-old WT, 31-month-old fbxl4−/− mutant zebrafish. *p < 0.05, **p < 0.01. |