Drug testing in zebrafish model of endotexemia. A, Methodology for zebrafish experiments. Zebrafish are treated with a lethal dose of lipopolysaccharide or maintained in control embryo medium at 3 (days postfertilization) dpf and examined for survival at 4, 5, and 6 dpf. B, Reverse transcriptase polymerase chain reaction (RT-qPCR) of cholesterol related genes from lipopolysaccharide-treated fish versus controls 3 hours after treatment at 3 dpf. Data represented as fold change lipopolysaccharide/controls. A value of 1 would signify no change, value greater than 1 is up-regulation in lipopolysaccharide treated versus controls, and value less than 1 is down-regulation. Individual dots represent separate experiments. The graph bars represent mean and sd. C, Differential expression analysis of RNA sequencing (RNA-seq) data from three lipopolysaccharide-treated zebrafish and three controls identified 12 lipid metabolism genes that were up-regulated in lipopolysaccharide-treated zebrafish compared with controls. D, Overlap of significantly differentially expressed genes between derivation, validation, and zebrafish groups.