Proliferation and apoptosis in the livers of male and female xmrk+ and Myc+ fish following oncogene activation. 10 fish were analysed in each group and the experiment was repeated multiple times. Proliferation and apoptosis were examined by PCNA and Caspase 3 staining respectively. (A) IF staining of PCNA in liver sections. (B) Quantification of densities of proliferating liver cells (PCNA+). (C) IF staining of Caspase-3 in liver sections. (D) Quantification of densities of apoptotic liver cells (Caspase 3+). *P < 0.05. Scale bars: 20 μm.

Immunofluorescent staining for cortisol and Tgfb1a in the livers of male and female xmrk+ and Myc+ fish following oncogene activation. 10 fish were analysed in each group and the experiment was repeated once for reproducibility. (A) IF co-staining of cortisol (red) and HNF4a (green) in liver sections. (B) Quantification of ratio of cortisol-expressing hepatocytes in liver sections. (C) IF co-staining of Tgfb1a (red) and HNF4a (green) in liver sections. (D) Quantification of ratio of Tgfb1a-expressing hepatocytes in liver sections.

Acknowledgments
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