Twist1a-ER^T2/xmrk double transgenic zebrafish offers an in vivo drug screening platform for the identification of anti-metastatic drugs.

A. Representative images of the dissemination of mCherry-labeled hepatic cells from the liver in Twist1a-ER^T2/xmrk double transgenic zebrafish at 16 days post-fertilization (dpf). The fish were treated with doxycycline and 4-hydroxytamoxifen (4-OHT). Some of the disseminated mCherry-positive cells are indicated by arrowheads. The images are shown as Z-stack images using 100× magnification. Scale bar, 200 μm. B. Representative images of the effect of adrenosterone on the dissemination of mCherry-positive cells in the fish at 16 dpf. Fish were treated with either vehicle (left) or adrenosterone (right). The images are shown as Z-stack images using 100× magnification. Scale bar, 100 μm. C. Mean frequencies of the fish showing the dissemination patterns of mCherry-positive cells in the vehicle- or adrenosterone-treated groups. Each value is presented as mean ± SEM from three independent experiments. Images are reprinted from Nakayama et al. (2020).

A Twist1a-ER^T2/xmrk double transgenic zebrafish expresses green fluorescent protein (GFP) in a skin-specific manner (A) and mCherry in a liver-specific manner (B).

Representative images of GFP and mCherry signals in Twist1a-ER^T2/xmrk double transgenic zebrafish at 6 days post-fertilization (dpf). Scale bar, 200 μm.