- Title
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Early-life exposure to environmentally relevant concentrations of triclocarban impairs ocular development in zebrafish larvae
- Authors
- Caioni, G., Merola, C., Bertolucci, C., Lucon-Xiccato, T., Savaşçı, B.B., Massimi, M., Colasante, M., Fioravanti, G., Cacciola, N.A., Ippoliti, R., d'Angelo, M., Perugini, M., Benedetti, E.
- Source
- Full text @ Chemosphere
Fig. 1. Results of the analysis performed on 4-dpf larvae. a) Representative images of zebrafish eyes with haematoxylin and eosin staining. Framed in red CMZ. 0,01% DMSO is the Solvent control. Scale bar 20 μm. b) Magnification of CMZ to appreciate the morphological changes. Scale bar 7 μm. c) Retinal organization in 4-dpf larvae. d) Measure of retina layers thickness. Statistical differences are indicated as * p < 0.05, **p < 0.005 and ***p < 0.0005 vs. DMSO, or as ##p < 0.005 vs. TCC 5. e) Magnification of RGL to appreciate the cell organization. Scale bar 7 μm. f) Cell density in RGL, measured as number of cells/area (μm2). Statistical differences are indicated as ***p < 0.0005 vs. solvent control. (The results are expressed as mean ± SEM). Abbreviations: SV, solvent control; CMZ, ciliary marginal zone; RPE, retinal pigment epithelium; ONL, outer nuclear layer; OPL outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; RGL, retinal ganglion cell layer. |
Fig. 2. Gene expression analysis of mitfb and pax6a. Statistical differences are indicated as * p < 0.05 vs. solvent control. (The results are expressed as mean ± SEM). |
Fig. 3. a) Results of the analysis performed on 20-dpf larvae. a) Representative images of zebrafish eyes. b) Representative images of zebrafish eyes with haematoxylin and eosin staining. Scale bar 20 μm. RPE, retinal pigment epithelium; PRL, photoreceptor layer; ONL, outer nuclear layer; OPL outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; RGL, retinal ganglion cell layer. c) Retinal organization in 20-dpf larvae. d) Measure of retina layers thickness. Statistical differences are indicated as * p < 0.05 vs. solvent control (SV). The results are expressed as mean ± SEM. |
Fig. 4. a) Gene expression analysis of mitfb and pax6a. Statistical differences are indicated as * p < 0.05 vs. solvent control. (The results are expressed as mean ± SEM). b) Visual discrimination performance of the subjects measured as preference for the circle stimulus versus the triangle stimulus (mean ± standard error) in the behavioral test. Asterisks indicate significant deviance from random choice (0.5 preference for the circle). *p < 0.05. |