FIGURE SUMMARY
Title

Honokiol Microemulsion Causes Stage-Dependent Toxicity Via Dual Roles in Oxidation-Reduction and Apoptosis through FoxO Signaling Pathway

Authors
Li, H., Li, W., Li, J., Li, S., Kuang, L., Pang, F., Jiang, H., Jin, H., Bian, X.
Source
Full text @ Cells
Figure 1

Chemical structure of honokiol.
Figure 2

Relationship between the honokiol microemulsion exposure period and the toxic response. (A) Survival rates of zebrafish embryos at 24, 48, 72 and 96 hpf. (B) Hatching rates of zebrafish embryos at 72 and 96 hpf. (C) Malformation rates of zebrafish at 96 hpf. The data are expressed as the mean ± SD (* p < 0.05, ** p < 0.01 compared with control embryos).
Figure 3

Measurement of the effect of the honokiol microemulsion on oxidative stress in zebrafish embryos. The content of oxidation products is shown in (A). The antioxidant activity is shown in (B). The mRNA expression of oxidative genes is shown in (C). The data are expressed as the mean ± SD (* p < 0.05, ** p < 0.01 compared with the control group).
Figure 4

Representative images of whole-embryo cell death determined by acridine orange staining at 24 hpf (A), 48 hpf (B), 72 hpf (C), and 96 hpf (D) in the control and honokiol microemulsion-exposed groups. The red boxes indicate representative apoptotic cells in brain.
Figure 4

Representative images of whole-embryo cell death determined by acridine orange staining at 24 hpf (A), 48 hpf (B), 72 hpf (C), and 96 hpf (D) in the control and honokiol microemulsion-exposed groups. The red boxes indicate representative apoptotic cells in brain.
Figure 5

mRNA expression of apoptosis-related genes and regulatory genes in 24 and 96 hpf embryos after honokiol microemulsion treatment. The mRNA expression levels of antiapoptotic and proapoptotic genes are shown in (A). The mRNA expression levels of apoptosis regulatory FoxO subtypes are shown in (B). The data are expressed as the mean ± SD (** p < 0.01 compared with control).
Figure 6

Dual roles of the honokiol microemulsion in oxidation-reduction in PC12 cells. (A) Cell growth curves of PC12 cells at different inoculation concentrations. (B) Effects of honokiol microemulsion on the viability of PC12 cells. (C) Effect of different toxic doses of honokiol microemulsion on the activity of PC12 cells. (D) Activity of SOD in PC12 cells exposed to different toxic doses of the honokiol microemulsion. (E) Effects of H2O2 on the viability of PC12 cells. Protective effects of the honokiol microemulsion on the cell viability (F) and SOD activity of PC12 cells injured by H2O2 (G). The data are expressed as the mean ± SD (** p < 0.01 compared with the control group; ## p < 0.01 compared with the H2O2 model group).
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cells
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