Behavioral profile of zebrafish larvae exposed to PTZ. The following graphs displays the average large count (y-axis) of larvae. (A) The average larval large count is depicted per 15 min interval (x-axis) of the tracking session. Zebrafish larvae were tested PTZ concentrations at 5 mM, 10 mM, 20 mM, and 40 mM (n = 70 fish/solution/concentration). Embryo medium (EM) was used as the control. Significance indicated by *p < 0.05 PTZ vs. EM, two-way ANOVA, Tukey’s post-hoc. (B) The total large count (y-axis) over 90 min is displayed. The respective treatment groups (x-axis). Analyses (one-way ANOVA, Tukey’s post-hoc) are marked * (p < 0.05) to indicate significant differences to embryo medium (EM).

Behavioral profile of zebrafish larvae exposed to carbamazepine (A) displays the average large count (y-axis) of larvae. The average larval large count is depicted per 15 min interval (x-axis) of the tracking session. The respective treatment groups are listed on the x-axis. Analyses (two-way ANOVA, Tukey’s post-hoc) are marked * (p < 0.05) to indicate significant differences to embryo medium (EM). Standard error of the means is shown. Analyses (two-way ANOVA, Tukey’s post-hoc) are marked and (p < 0.05) to indicate significant differences to 10 mM PTZ. (B) The total large count (y-axis) over 90 min is displayed. The respective treatment groups (x-axis). Analyses (one-way ANOVA, Tukey’s post-hoc) are marked * (p < 0.05) to indicate significant differences to embryo medium (EM). Standard error of the means is shown. Analyses (one-way ANOVA, Tukey’s post-hoc) are marked & (p < 0.05) to indicate significant differences to 10 mM PTZ. (n = 76 fish/treatment group).

Behavioral profile of zebrafish larvae exposed to lamotrigine. (A) The average large count (y-axis) of larvae is depicted per 15 min interval (x-axis) of the tracking session. The respective treatment groups are listed on the x-axis. Analyses (two-way ANOVA, Tukey’s post-hoc) are marked * (p < 0.05) to indicate significant differences to embryo medium (EM). Standard error of the means is shown. Analyses (two-way ANOVA, Tukey’s post-hoc) are marked & (p < 0.05) to indicate significant differences to 10 mM PTZ. (B) The total large count (y-axis) over 90 min is displayed. The respective treatment groups (x-axis). Analyses (one-way ANOVA, Tukey’s post-hoc) are marked * (p < 0.05) to indicate significant differences to embryo medium (EM). Standard error of the means is shown. Analyses (one-way ANOVA, Tukey’s post-hoc) are marked & (p < 0.05) to indicate significant differences to 10 mM PTZ. (n = 76 fish/treatment group).

Behavioral profile of zebrafish larvae exposed to topiramate. (A) The average large count (y-axis) of larvae is depicted per 15 min interval (x-axis) of the tracking session. The respective treatment groups (x-axis). Analyses (two-way ANOVA, Tukey’s post-hoc) are marked * (p < 0.05) to indicate significant differences to embryo medium (EM). Standard error of the means is shown. Analyses (two-way ANOVA, Tukey’s post-hoc) are marked & (p < 0.05) to indicate significant differences to 10 mM PTZ. (B) The total large count (y-axis) over 90 min is displayed. The respective treatment groups (x-axis). Analyses (one-way ANOVA, Tukey’s post-hoc) are marked * (p < 0.05) to indicate significant differences to embryo medium (EM). Standard error of the means is shown. Analyses (one-way ANOVA, Tukey’s post-hoc) are marked & (p < 0.05) to indicate significant differences to 10 mM PTZ. (n = 91 fish/treatment group).

Behavioral profile of zebrafish larvae exposed to GS967. (A) The average large count (y-axis) of larvae. The average larval large count is depicted per 15 min interval (x-axis) of the tracking session. The respective treatment groups (x-axis). Analyses (two-way ANOVA, Tukey’s post-hoc) are marked * (p < 0.05) to indicate significant differences to embryo medium (EM). Standard error of the means is shown. Analyses (two-way ANOVA, Tukey’s post-hoc) are marked & (p < 0.05) to indicate significant differences to 10 mM PTZ. (B) The total large count (y-axis) over 90 min is displayed. The respective treatment groups (x-axis). Analyses (one-way ANOVA, Tukey’s post-hoc) are marked * (p < 0.05) to indicate significant differences to embryo medium (EM). Standard error of the means is shown. Analyses (one-way ANOVA, Tukey’s post-hoc) are marked & (p < 0.05) to indicate significant differences to 10 mM PTZ. (n = 60 fish/treatment group).

Calcium signaling activity in 7 dpf larvae. The fluorescence score is displayed on the y-axis. The respective treatment groups are shown on the x-axis. Analyses (one-way ANOVA, Tukey’s post-hoc) are marked * (p < 0.05) to indicate significant differences to embryo medium (EM). Standard error of the means is shown. Analyses (one-way ANOVA, Tukey’s post-hoc) are marked & (p < 0.05) to indicate significant differences to 10 mM PTZ. (n = 12 fish/treatment group).

Local field potentials in 7 dpf larvae. (A) Representative tracings recorded in the presence of EM (top) and 10 mM PTZ (bottom) are shown. (B) The relative LFP difference is displayed on the y-axis. The respective treatment groups are shown on the x-axis. Analyses (one-way ANOVA, Tukey’s post-hoc) are marked * (p < 0.05) to indicate significant differences to embryo medium (EM). Standard error of the means is shown. Analyses (one-way ANOVA, Tukey’s post-hoc) are marked & (p < 0.05) to indicate significant differences to 10 mM PTZ. (n = 8 fish/treatment group).