Fig. 1

Fig. 1. BAcAm-induced developmental toxicity in zebrafish embryos. [A and B] Mortality rate, [C] hatching rate, and [D] body length. [E] Morphological damage to embryos. Abbreviations: pericardial edema (Pe), spine deformation (Sd), yolk sac edema (Yse), tail malformation (Tm). [F] Malformation rate. Values are the mean ± SEM. (*P < 0.05, **P < 0.01 and ***P < 0.001).

Fig. 2

Fig. 2. BAcAm decreased the locomotor ability of embryos. [A] Movement distance of zebrafish in each group with light and 10% dim light (30 lux.) alternately provided every 20 min (the white boxes in the x axis indicate periods of light; the black boxes indicate periods of darkness). [B & C] Total movement distance of zebrafish in light and dark conditions. Values are the mean ± SEM. (*P < 0.05).

Fig. 3

Fig. 3. BAcAm enhanced T3, T4 and TSH secretions. [A] T3, [B] T4, [C] the ratio of T3/T4, and [D] TSH. Values are the mean ± SEM. (**P < 0.01 and ***P < 0.001).

Fig. 4

Fig. 4. Effects of BAcAm on zebrafish tshβ mRNA expression. [A] EGFP expression in the pituitary gland of tshβ: EGFP transgenic fish (ventral views). [B] Quantitative analysis of EGFP integrated densities (IDs). [C] Whole in-situ hybridization showed the endogenous tshβ mRNA expression in the pituitary of WT zebrafish (ventral views). [D] Quantitative analysis of whole in-situ hybridization IDs. Values are the mean ± SEM. (***P < 0.001).

Fig. 5

Fig. 5. Transcriptional changes in TH regulating genes following BAcAm treatments. Expression of HPT axis-related genes [A] trh, [B] tshr, [C] tg, [D] nis, [E] tpo, [F] dio1, [G] dio 2, [H] tr α, [I] tr β, [J] ugt1ab. Upregulated by T3 genes [K] klf9 and [L] rho. Values are the mean ± SEM. (*P < 0.05, **P < 0.01 and ***P < 0.001).
Fig. 6

Fig. 6. BAcAm-induced toxic effects were rescued via injection of capped tr β mRNAs. [A] Mortality rate; [B] hatching rate; [C] malformation rate; [D] body length; [E] movement distance of embryos; contents of [F] T3, [G] T4, [H] TSH; and [I] the T3/T4 ratio. Values are the means ± SEM. Different letters (a–c) indicate significant differences.
Acknowledgments
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