FIGURE SUMMARY
Title

Liposomal Clodronate-mediated Macrophage Depletion in the Zebrafish Model

Authors
Yang, L., Rojas, A.M., Shiau, C.E.
Source
Full text @ Bio Protoc
Fig. 1

A. Capillary tube loaded into micro-needle puller. B. Magnified view of heated filament surrounding glass capillary. C. Creation of 2 microinjection needles from pulling (double-sided white arrow).

Fig. 2

A. Mounted larvae are placed under a fluorescent stereomicroscope adjacent to a microinjection needle attached to a micro-manipulator. B. Higher magnification of mounted larvae. Dotted circle shows solidified thin layer of low-melt agarose covered with a small pool of system water supplemented with PTU. Black arrows point to larvae. Red arrow points to tip of needle (Note: This arrow is not pointing to the injection site.)

Fig. 3

A. Color of fish water after addition of neutral red. B. Color of fish and water after removal of neutral red. C. Full body image of control uninjected 4 dpf larvae after neutral red staining. D and E. Lateral (D) and dorsal (E) view of neutral red staining. C-E. Larvae imaged in 3% methyl cellulose. Black arrows point to individual microglial cells.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Bio Protoc
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