A Volcano plots for whole transcriptomes of differentially expressed genes between KO and WT MEFs. B Venn diagrams illustrating the overlap of the differentially expressed genes (down- or upregulated genes, FC ≤ −1,5 or ≥1,5, pval_adj≤0.05) between KO and WT MEFs, under heat shock. C Gene set enrichment analysis barcode plot of transcriptomic changes of “Cellular response to heat stress” and “regulation of HSF1 mediated heat shock” (from Reactome database) in KO MEFs compared with WT MEFs. Red, blue, and pink shaded rectangles mark upregulated, downregulated, and genes that are unchanged, respectively. D Transient knockdown of the putative TG2 paralogous was performed with three independent morpholinos against zTg2a, zTg2b, and zTg2l; a standard control morpholino was also used. Bright-field images of representative morphants are reported. (n = 50). Bars: 1 mm. E Double transgenic Tg(-1.5hsp70l:GAL4^(+/−); UAS:Kaede^(+/−)) were injected at one-cell stage either with Tg2bMO or CtrlMO, heat-shock inductions were performed at 24 hpf by incubation of larvae for 1 h at 37 °C, whereas images were acquired between 29 and 30 hpf. Heat-shock protein activation was followed by an increase in the fluorescence. Bright-field and epifluorescent images are reported. The images are representative of different experiments. GFP epifluorescence quantification is reported in the graph on the right. Dots represent treated embryos (n of embryos >40). Thapsigargin was used as a positive control. (n = 3; means±SEM; *p < 0.05; ***p < 0.001). Bars: 1 mm.