CRISPR

CRISPR4-tmc1

ID

ZDB-CRISPR-210104-1

Name

CRISPR4-tmc1

Previous Names

None

Target

tmc1 (1)

Sequence

5' - TCAGAGAAAAGAACAAACGA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
vo69	tmc1
vo70	tmc1
vo71	tmc1

Expression

Gene expression in Wild Types + CRISPR4-tmc1

No data available

Phenotype

Phenotype resulting from CRISPR4-tmc1

No data available

Phenotype of all Fish created by or utilizing CRISPR4-tmc1

Phenotype	Fish	Conditions	Figures
detection of mechanical stimulus involved in sensory perception of sound decreased efficacy, abnormal	tmc2a^vo72/vo72; tmc1^vo69/vo69	standard conditions	Fig. 7 from Smith et al., 2020
vestibular reflex decreased occurrence, abnormal	tmc2b^{sa8817/sa8817}; tmc1^vo69/vo69	standard conditions	Fig. 9 from Smith et al., 2020
auditory behavior decreased occurrence, abnormal	tmc2b^{sa8817/sa8817}; tmc2a^vo72/vo72; tmc1^vo69/vo69	standard conditions	Fig. 1 from Smith et al., 2020
vestibular reflex decreased occurrence, abnormal	tmc2b^{sa8817/sa8817}; tmc2a^vo72/vo72; tmc1^vo69/vo69	standard conditions	Fig. 1, Fig. 5 from Smith et al., 2020
swim bladder uninflated, abnormal	tmc2b^{sa8817/sa8817}; tmc2a^vo72/vo72; tmc1^vo69/vo69	standard conditions	Fig. 1 from Smith et al., 2020
response to auditory stimulus disrupted, abnormal	tmc2b^{sa8817/sa8817}; tmc2a^vo72/vo72; tmc1^vo69/vo69	standard conditions	Fig. 4, Fig. 6 from Smith et al., 2020
response to auditory stimulus normal occurrence, ameliorated	tmc2b^{sa8817/sa8817}; tmc2a^vo72/vo72; tmc1^vo69/vo69; vo28Tg	standard conditions	Fig. 6 from Smith et al., 2020
vestibular reflex normal occurrence, ameliorated	tmc2b^{sa8817/sa8817}; tmc2a^vo72/vo72; tmc1^vo69/vo69; vo28Tg	standard conditions	Fig. 5 from Smith et al., 2020
anterior macula response to auditory stimulus normal occurrence, ameliorated	tmc2b^{sa8817/sa8817}; tmc2a^vo72/vo72; tmc1^vo69/vo69; vo28Tg	standard conditions	Fig. 5, Fig. 6 from Smith et al., 2020
lateral crista response to auditory stimulus normal occurrence, ameliorated	tmc2b^{sa8817/sa8817}; tmc2a^vo72/vo72; tmc1^vo69/vo69; vo28Tg	standard conditions	Fig. 5 from Smith et al., 2020

Citations