- Title
-
Establishing a new animal model for muscle regeneration studies
- Authors
- Pourghadamyari, H., Rezaei, M., Ipakchi-Azimi, A., Eisa-Beygi, S., Basiri, M., Tahamtani, Y., Baharvand, H.
- Source
- Full text @ Mol Biol Res Commun
Evalute the expression of myod, myf5, and pax7 in muscle regeneration process. |
The workflow of the study. Zebrafish embryos were microinjected at one-cell-stage with the desired gene construct. Two days after injection, embryos were evaluated for CFP expression. CFP positive embryos were selected to raise as F0 embryos transgenic fish. CFP positive embryos were selected and raised. CFP positive adult zebrafishes were crossed with wild type (TU strain) to produce the F1 generation. |
The CFP of Tg(mylpfa:cfp-nfsB) is specifically expressed in muscle cells. |
MTZ treatment specifically depletes muscle cells of Tg(mylpfa:cfp-ntr) and muscle regeneration. |
Graph of relative CFP intensities at 3 dpf, 5 dpf and 8dpf. *P<0.05 and **P<0.01, n = 6. (dpf=Days Post Fertilization). |