Protective effect of AP-1 and AP-4 in LPS-induced zebrafish. (A) AP-1 and AP-4 reduced the mortality of zebrafish after LPS injection. The yolks of larvae were microinjected with 2 mg/mL LPS at 3 days post fertilization (dpf), and mortality was monitored for 72 h. Negative controls were microinjected with phosphate-buffered saline (PBS). Data are represented as the mean of three independent experiments. ^## p < 0.01 versus control group, ** p < 0.01 versus model group by one-way ANOVA with Tukey’s test. (B) AP-1 and AP-4attenuated the histopathological changes in LPS-stimulated zebrafish. The zebrafish were dehydrated and embedded in paraffin. Then, paraffin sections (4 μm) were stained by H&E. The black arrows represent inflammatory cells.

AP-1 and AP-4 alleviated the inflammatory response in zebrafish subjected to LPS microinjection. (A) Representative images of zebrafish in LPS-induced inflammation model. (B) Neutrophils in the region of interest (red circles) were quantitatively analyzed. Data are represented as the mean ± SE of three independent experiments. ^## p< 0.01 versus control group, ** p < 0.01 and * p < 0.05 versus model group by one-way ANOVA with Tukey’s test.