- Title
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The ezh2(sa1199) mutant zebrafish display no distinct phenotype
- Authors
- San, B., Rougeot, J., Voeltzke, K., van Vegchel, G., Aben, M., Andralojc, K.M., Flik, G., Kamminga, L.M.
- Source
- Full text @ PLoS One
ezh2(sa1199) zebrafish line has non-specific phenotypes. A. Whole mount in-situ hybridization for ezh2 on 3 dpf ezh2(sa1199) (left panel) and ezh2(hu5670) (right panel) embryos. Left panel. Wild type siblings (top) show ezh2expression in the brain, eyes, branchial arches, and the gut (white arrowheads). Heterozygotes (middle) and ezh2(sa1199) mutants (bottom) show the same expression pattern; they are phenotypically comparable to wild types and have the same spatiotemporal ezh2 expression pattern. Right panel. Wild type siblings (top) show similar ezh2 expression to ezh2(sa1199) in-cross embryos (white arrowheads). Heterozygotes (middle) show decreased expression, and ezh2 is barely detectable in ezh2(hu5670) mutants (bottom). Scale bar: 500 μm. Numbers indicate the number of embryos displaying the shown expression pattern per total number of embryos tested. B. Left panel shows normal wild type and mutant embryos at 3 and 2 dpf, respectively. The right panel shows 2 and 3 dpf wild type and mutant embryos, respectively, with yolk sac, heart, and brain edemas (white arrowheads) and spinal curvatures (black arrowheads). EXPRESSION / LABELING:
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Selectively out-crossed ezh2(sa1199) survives until adulthood. A. Survival graph of ezh2(sa1199). Survival assay was performed with ezh2(sa1199) in-crossed wild type (N = 18, blue line) and mutant (N = 21, orange line) siblings until 46 dpf. Some larval lethality during raising was seen both in wild types and mutants. The graph indicates that the survival of homozygous ezh2(sa1199) mutants is not significantly different from wild type siblings. B. Pictures of wild type and mutant ezh2(sa1199) adult siblings. While wild types have normal jaws (left), adult ezh2(sa1199)mutants (right) display an open mouth phenotype (black arrowhead). C. RT-qPCR measurement of ezh1 and ezh2 mRNA in ezh2(sa1199) adults. The (decapitated) body of 70 dpf wild type (blue) and mutant (orange) individuals were lysed and the presence of ezh1 and ezh2 mRNA was quantified and normalized to the reference genes ß-actinand ef1a. The ezh1 (p = 0.9509) and ezh2 (p = 0.1493) mRNA levels were not significantly altered in mutants. D. Ezh2 protein in single 70 dpf adults and H3K27me3 in 7 dpf pooled larvae. The presence of Ezh2 was tested in wild type (N = 3, left) and mutant (N = 4, right) bodies at 70 dpf, where Ezh2 expression persists (upper panel). At 7 dpf, mutant larvae (right) show 41.8% decreased levels of the H3K27me3 mark compared to wild type (left) larvae (lower panel) and relative to β-actin loading control. For each Western blot, representative bands were cropped from the same blot and shown in boxes for clarity of presentation. EXPRESSION / LABELING:
PHENOTYPE:
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. EXPRESSION / LABELING:
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