- Title
-
Impaired Liver Size and Compromised Neurobehavioral Activity are Elicited by Chitosan Nanoparticles in the Zebrafish Embryo Model
- Authors
- Abou-Saleh, H., Younes, N., Rasool, K., Younis, M.H., Prieto, R.M., Yassine, H.M., Mahmoud, K.A., Pintus, G., Nasrallah, G.K.
- Source
- Full text @ Nanomaterials (Basel)
(A) Acute toxicity representative pictures obtained with a stereomicroscope at magnification X = 0.63. Note the deformed embryos in dimethylaminobenzaldehyde (DEAB): short size, scoliosis heart edema (closed arrow), and yolk edema (open arrow). (B) Cumulative survival/mortality. (C) Mortality response curve of different concentrations of DEAB and ChNPs. (D) Teratogenic phenotypes analysis. DEAB: Dimethylaminobenzaldehyde, positive control for toxicity and teratogenicity. |
(A) Representative images for the RFP liver area after each treatment. (B) Quantification and measurement of the RFP liver area (mm2) for the controls and all the tested compounds. (C) Representative images for steatosis and yolk retention. Arrow (↗) in EtOH treatment indicate liver steatosis (absorbed ORO stain by the liver), and arrows (↗) in paracetamol (APAP) and ChNP treatments indicate increased yolk retention (absorbed ORO stain by the yolk). (D) Percentages of embryos showed yolk retention and (E) steatosis. Thirty embryos were used for each treatment in every experiment. *, significantly different from DMSO (p < 0.05); **, significantly different from DMSO (p < 0.01). |