- Title
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UBTOR/KIAA1024 regulates neurite outgrowth and neoplasia through mTOR signaling
- Authors
- Zhang, H., Zhang, Q., Gao, G., Wang, X., Wang, T., Kong, Z., Wang, G., Zhang, C., Wang, Y., Peng, G.
- Source
- Full text @ PLoS Genet.
Disruption of ubtor gene increases mTOR signaling in zebrafish brain tissues. (A) Immunoblot analysis of p-S6K levels in wild type (wt) controls and ubtor-/- mutants. The head tissues were isolated from 5 dpf zebrafish before introducing food to fish larvae. For quantitative analysis, the p-S6K/S6K values were normalized to that of wt. t = 9.840, df = 2, P = 0.0102. Representative results from three biological repeats. (B) Immunoblot analysis of mTOR signaling in wt controls and ubtor-/- mutants under fasted and re-fed conditions. Zebrafish larvae of 13.5 dpf were starved for 36 hours, and then the larvae in the re-fed group were fed for 12 hours. Brain tissues were dissected out from the fasted and the re-fed larvae. For quantitative analysis, the p-S6K/S6K and p-S6/S6 values were normalized to that of wt. For p-S6K/S6K, t = 5.089, df = 2, P = 0.0385 under the fasted condition. For p-S6/S6, t = 4.637, df = 2, P = 0.0435 under the fasted condition, and t = 6.077, df = 2, P = 0.0260 under the re-fed condition. Representative results from three biological repeats. (C) Dominant-active HRAS(G12V) induced tumor formation in wild type controls (wt) and ubtor mutants. Three and four biological repeats for the wt and the mutant, respectively. t = 4.343, df = 5, P < 0.01. n = 59 and 37 for the wt and the mutant, respectively. χ12 = 13.23, P < 0.001. EXPRESSION / LABELING:
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ubtor/kiaa1024 gene mapping and expression. (A) Transgenic expression of EGFP in a 5 dpf larva from the cross between the enhancer trap line Tg(ubtor:GAL4FF) and the reporter line Tg(UAS:EGFP). Larva was embedded in agarose and imaged with a confocal microscope. Five image fields were stitched together to show the full length of the animal. (B) Genomic sequences at the insertion site in the Tg(ubtor:GAL4FF) line. The Tol2-GAL4FF transgene is inserted at the second intron in the ubtor gene. (C) Phylogeny tree of Ubtor genes in various vertebrate species. No Ubtor homologs were found outside the vertebrates. (D) EGFP expression in the brain region in the ubtor enhancer trap line. Ha: habenula. Dorsal View. (E-F) In situ hybridization analysis of endogenous ubtor gene expressions at 3 dpf and 5 dpf. Dorsal View. Scale bars, 200 μm. EXPRESSION / LABELING:
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. PHENOTYPE:
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