FIGURE SUMMARY
Title

Adaptable, illumination patterning light sheet microscopy

Authors
Power, R.M., Huisken, J.
Source
Full text @ Sci. Rep.
Fig. 5

Illumination patterning in live zebrafish larvae (42% TAG amplitude). Scale bars = 100 μm. (a,b) Tg(kdrl:GFP) zebrafish (5 dpf), (c,d): Tg(h2afva:h2afva-EGFP) (5 dpf). (a) Fully illuminated plane through head and trunk section. (b) Patterning along x by phase-delayed illumination pulsing. (c) Fully illuminated plane through head and trunk section. Cyan box corresponds to ROI for (d). (d) Patterning along x by phase-delayed illumination pulsing. White line shows y-averaged illumination intensity. Red line shows position of beam focus for calibration performed in fluorescent dye solution.
Fig. 6

Image quality (Tg(kdrl:GFP), 5 dpf) and photobleaching (Tg(h2afva:h2afva-EGFP), 5 dpf) in zebrafish (42% TAG amplitude). All images are maximum intensity projections along the excluded axis. Scale bars = 100 μm. (a) 1D scanned mode, 0.1 mW laser power. (b) 2D swept/scanned mode, 0.5 mW laser power.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.
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