FIGURE SUMMARY
Title

Comparison of solvate ionic liquids and DMSO as an in vivo delivery and storage media for small molecular therapeutics

Authors
Yoganantharajah, P., Ray, A.P., Eyckens, D.J., Henderson, L.C., Gibert, Y.
Source
Full text @ BMC Biotechnol.
Fig. 2

Room temperature DEAB exposure in developing zebrafish embryos. Embryos exposed to DEAB at 5 μM in solution at room temperature for 4 months in either DMSO (b), G3TFSA (c), or G4TFSA (d) displayed the reported characteristic of loss of RA (compared to control (DMSO exposed only (a): lack of pectoral fin induction (arrowhead), shortening of the posterior head (asterisk), malformation of the otic vesical (arrow) and pericardial edema
Fig. 3

Frozen DEAB exposure in developing zebrafish embryos. Embryos exposed to DEAB at 5 μM that have been stored for 4 months at -20 °C in either DMSO (b), G3TFSA (c), or G4TFSA (d) display the reported characteristics of loss of RA (compared to control (DMSO exposed only (a): lack of pectoral fin induction (arrowhead), shortening of the posterior head (asterisk), malformation of the otic vesical (arrow) and pericardial edema
Fig. 4

SU5402 exposure in developing zebrafish embryos. Embryos are exposed to SU5402 (d) at 2.5 μM (b & c) or 5 μM (e & f) in either DMSO or G4TFSA display the reported characteristics of FGF signaling inhibition compared to control (untreated (a) or DMSO exposed only (b & e)): lack of pectoral fin induction (marked by an asterisk), malformation of the otic vesicle (arrow). Lloss of MHB (open arrow head) was observed in embryos treated with 5 μM DEAB. 100× magnification
Fig. 5

Expression of dlx2a visualized using whole mount in situ hybridization. Embryos exposed to SU5402 at 2.5 μM in either DMSO (b & e) or G4TFSA (c & f) display reduced localization and expression of dlx2a compared to control (DMSO exposed only (a & d) in the hindbrain (arrow) and in the pharyngeal arches (arrow head, * lack of pharyngeal arches). The first row depicts the embryos in a later orientation, the second row depicts the embryos in a ventral orientation
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Biotechnol.
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