FIGURE SUMMARY
Title

Depdc5 knockdown causes mTOR-dependent motor hyperactivity in zebrafish

Authors
de Calbiac, H., Dabacan, A., Marsan, E., Tostivint, H., Devienne, G., Ishida, S., Leguern, E., Baulac, S., Muresan, R.C., Kabashi, E., Ciura, S.
Source
Full text @ Ann Clin Transl Neurol
Fig. 1

depdc5 expression in early development in the zebrafish larva. (A) Ventral view of a 28 hpf embryo showing expression of the depdc5 transcript in the telencephalon. (B) In situ hybridization using the control probe showing no positive signal. (C and D) Lateral and dorsal views, respectively, of the 28 hpf embryo showing the expression pattern in the brain and spinal cord. (E) RT‐PCR detecting Depdc5 expression in tissue obtained from zebrafish larvae at different stages of development.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Shield to Protruding-mouth
Fig. 2

Depdc5 knockdown larvae show an early hyperactive motor phenotype. (A) Locomotor activity heatmap illustrating spontaneous movement in 28 hpf larvae inside the chorion over a period of 1 min showing an increase in general movement in the Depdc5 knockdown (KD) larvae as compared to mismatch and noninjected controls. (B) Parameters of the same activity showing increased frequency and amplitude of movement in Depdc5 KD animals. C. Quantification of total activity showing a significant hyperactive phenotype in the Depdc5 KD. Both the coiling and twitching frequency were significantly increased as compared to controls.

PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5
Fig. 3

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec
Fig. 4

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Day 4 to Day 6
Fig. 6

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. S1

Depedc5 knockdown does not affect the gross morphology of zebrafish embryos.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Ann Clin Transl Neurol
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