- Title
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Mutation of IPO13 causes recessive ocular coloboma, microphthalmia, and cataract
- Authors
- Huang, X.F., Xiang, L., Cheng, W., Cheng, F.F., He, K.W., Zhang, B.W., Zheng, S.S., Han, R.Y., Zheng, Y.H., Xu, X.T., Yu, H.Y., Zhuang, W., Leung, Y.F., Jin, Z.B.
- Source
- Full text @ Exp. Mol. Med.
a Lateral view of zebrafish larvae. Larvae injected with the highest does of ipo13 targeting MO at 5.0 ng/µL exhibit apparent spinal curvature and reduced eye size. Abnormalities were not observed in the low concentration groups. b Magnified lateral pictures of zebrafish eyeballs show significant decrease in eye size. c Vertical view of larval eyeballs displays shorter axial length in the highest MO dose group. d, e Quantification of the eyeball axial length (d) and eye area (e). The axial length and eye area are represented as a white line and red circle, respectively. Student’s t-test, values are means ± s.e.m. ***P < 0.001 EXPRESSION / LABELING:
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Left panel shows the control MO 5.0 ng, ipo13-MO 1.0 ng, and ipo13-MO 2.5 ng injected larvae expressing zpr-1 in relative normal levels at 3 dpf. Less zpr-1 signal is detected in the peripheral area of larval retinas. The three nuclear layers are even in these three groups. Right panel shows that ipo13-MO 5.0 ng injection disturbs the lamination of retinal nuclear layers. Both plexiform layers are not observed in this group, and almost no zpr-1 expression is detected PHENOTYPE:
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. PHENOTYPE:
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a Lateral view of larvae injected with MO and/or rescue full-length mRNA. Larvae co-injected with MO and rescue mRNA display a relatively normal morphology that is similar to the morphology in the rescue mRNA only group on 3 dpf. Meanwhile, compared to the MO only group, the coloboma phenotype decreases significantly in the rescue group. b, c MO and mRNA co-injected larvae show dramatic recovery in both axial length (b) and eye area (c). Rescue experiments were repeated three times |
Knocking down ipo13 leads to cataract in zebrafish. Lateral view of 3dpf control MO (A) and ipo13-MO (B-D) injected larvae. The portion of cataract exhibiting larvae is 8/59. PHENOTYPE:
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ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions. PHENOTYPE:
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