Time-course physiological effect in newly hatched zebrafish larvae exposed to BDE-99 and BDE-47. Two-hour post-hatching (2 hph) larvae were exposed to BDE-99 and BDE-47 of different concentrations (50 nM and 500 nM) for 8 h, 24 h, 48 h, 72 h and 96 h. Paracardiac edema and yolk sac edema were observed in larvae exposed to BDE-99 and noted in solid line arrow and dotted line arrow, respectively. Images were captured from lateral view. All scale bars are 100 μm.

Yolk sac edema and pericardial edema in newly hatched zebrafish larvae exposed to BDE-99 of 500 nM at 96 h. Two-hour post-hatching (2 hph) larvae were exposed to BDE-99 of 500 nM for 96 h. Paracardiac edema and yolk sac edema were observed. Larvae was pictured at dorsal view (upper section) and lateral view (lower section) with scale bar of 100 μm.

In situ hybridization results of cyp1a mRNA in newly hatched zebrafish larvae. Larvae harvested 2 h after hatching were exposed to BDE-99 and BDE-47 of 500 nM for 24 h, as shown by blue coloration in in situ hybridization with whole mount. Larvae were captured in left view and dorsal view, respectively. The cyp1a probe was detected as shown (solid line arrow: liver; dotted line arrow: intestine). Sense probe was used as negative control. All scale bars are 100 μm.